野鼠色相关蛋白(agout-related protein,AGRP)是一种在人及129/SV小鼠下丘脑和肾上腺表达的蛋白质,它是黑皮质素受体2(Mc2r)和黑皮质素受体4(Mc4r)的有效拮抗剂,主要在肾上腺和中枢神经系统中表达,参与调控体重。作者分别利用非洲爪蟾属黑素细胞,转入黑皮质素受体的细胞株及AGRP转基因鼠,对AGRP的作用进行了进一步研究,实验分体外和体内进行。 体外实验包括将一定量的AGRP和α-MSH加 Agout-related protein (AGRP), a protein expressed in the hypothalamus and adrenal gland of human and 129 / SV mice, is a member of melanocortin receptor 2 (Mc2r) and melanocortin receptor 4 ( Mc4r) effective antagonist, mainly in the adrenal and central nervous system expression, involved in the regulation of body weight. The authors used the Xenopus melanocytes, the cell line transfected with melanocortin receptors and the AGRP transgenic mice respectively to further study the effect of AGRP. The experiments were carried out in vitro and in vivo. In vitro experiments include adding a certain amount of AGRP and α-MSH plus