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上皮生长因子(EGF)属于一类激素样细咆调节因子,可促进多种正常细胞及肿瘤细胞的体外增殖。上皮生长因子受体(EGFR)是一分子量为170 000—180 000道尔顿的细胞表面多肽,其组织学分布较广泛,在某些肿瘤细胞上有异常高的表达。如源自女性外阴部上皮癌的A431细胞和源自人乳腺癌的MDA-468。本研究报告了在源自人鼻咽部低分化鳞癌的细胞株CNE-2 Z上有较高的EGFR表达(1.3×10 10~5/细胞)。当EGF终浓度低予1×10~(-10)M,对A431细胞、CNF-2 Z细胞的体外增殖有着和缓的促进作用;当EGF的终浓度为1×10~(-9)M时,A431细胞和CNE细胞分别受抑制,而CNE-2 Z细胞仍处于促增殖状态;随着EGF浓度的进一步增高,CNE-2 Z亦转为受抑状态;当EGF浓度为1×10~(-7)M时,A431和CNE完全受抑制;CNE-2Z抑制率为61％。以上结果提示高浓度的EGF对CNE-2 Z的体外增殖具有抑制作用。
Epidermal growth factor (EGF) belongs to a class of hormone-like fine bream regulatory factors, which can promote the proliferation of a variety of normal cells and tumor cells in vitro. Epidermal growth factor receptor (EGFR) is a cell surface polypeptide with a molecular weight of 170 000-180 000 daltons. It has a wide histological distribution and abnormally high expression on some tumor cells. Such as A431 cells derived from female genital epithelial cancer and MDA-468 derived from human breast cancer. This study reported higher EGFR expression (1.3 x 10 10~5 cells/cell) in cell line CNE-2 Z derived from poorly differentiated squamous cell carcinoma of human nasopharynx. When the final concentration of EGF is lower than 1×10 -10 M, the proliferation of A431 cells and CNF-2 Z cells is promoted in vitro; when the final concentration of EGF is 1×10 -9 M , A431 cells and CNE cells were inhibited, while CNE-2 Z cells were still in the proliferative state; with the further increase of EGF concentration, CNE-2 Z also turned into a suppressed state; when the EGF concentration was 1 × 10 ~ ( -7)M, A431 and CNE were completely inhibited; the inhibition rate of CNE-2Z was 61%. These results suggest that high concentration of EGF can inhibit the proliferation of CNE-2 Z in vitro.