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目的:观察以鳖甲和三七为主要成分的复方鳖甲软肝片和氯沙坦对心肌成纤维细胞(cardiacfibroblasts,CFs)增殖的影响。方法:取生后1～3d的雄性SD仔鼠心脏原代培养心肌成纤维细胞、传代,将第3代心肌成纤维细随机分为3组:空白对照组、氯沙坦组、复方鳖甲软肝片组。并根据血清药理学理论,分别以蒸馏水、10mg/kg氯沙坦、0.7g/kg复方鳖甲软肝片喂养新西兰兔获得血清,制备相应培养液干预培养的心肌成纤维细胞。结果:鳖甲片和氯沙坦能明显抑制CFs增殖(空白对照组、氯沙坦组、鳖甲片组的A值分别为0.387±0.0539,0.181±0.044,0.212±0.038,F=5.805,P=0.016),并将细胞周期阻抑在G1期犤(74.32±1.76)%,(81.02±4.15)%,(81.62±3.83)%,F=7.041,P=0.009犦,处于分裂期(S期)的细胞比例减少犤(18.36±2.15)%,(13.18±3.26)%,(11.60±2.23)%,F=9.290,P=0.004犦,对CFs增殖和细胞周期影响的作用强度与氯沙坦相近(A值:95%CI:-0.180～1.118,P=0.662;G1和S期:95%CI:-5.306～4.106,P=0.768;95%CI:-1.995～5.515,P=0.355)。结论:复方鳖甲软肝片具有防治心肌纤维化的作用。
OBJECTIVE: To observe the effects of Fufang Biejiarangan Tablets and Losartan on the proliferation of cardiac fibroblasts (CFs) with the main components of Biejia and Panax notoginseng. Methods: Cardiac fibroblasts were primary cultured from male SD pups 1 to 3 days after birth and passaged. The third generation of cardiac fibroblasts were randomly divided into 3 groups: control group, losartan group and turtle Ruanpudan group. According to the theory of serum pharmacology, New Zealand rabbits were fed with distilled water, losartan 10mg / kg and 0.7g / kg Fufang Biejia Ruangan, respectively to obtain the serum, and the corresponding culture solution was used to prepare the cultured myocardial fibroblasts. Results: The turtle tablets and losartan significantly inhibited the proliferation of CFs (blank control group, losartan group, turtle tablet group A value were 0.387 ± 0.0539,0.181 ± 0.044,0.212 ± 0.038, F = 5.805, P = 0.016), and the inhibition of cell cycle in the G1 phase 犤 (74.32 ± 1.76)%, (81.02 ± 4.15)%, (81.62 ± 3.83)%, F = 7.041, P = 0.009 犦, ) Decreased (.3 18.36 ± 2.15)%, (13.18 ± 3.26)%, (11.60 ± 2.23)%, F = 9.290, P = 0.004 犦, respectively. The effect on the proliferation and cell cycle of CFs was stronger than that of losartan (A value: 95% CI: -0.180 to 1.118, P = 0.662; G1 and S phases: 95% CI: -5.306 to 4.106, P = 0.768; 95% CI: -1.995 to 5.515, P = 0.355). Conclusion: Fufang Biejia Ruangan Tablet can prevent myocardial fibrosis.