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自1973年培养巴西橡胶花药以来,已产生了诱导频率很高的愈伤组织和近4万个胚状体。在1977年底至1978年春先后从高产无性系“海垦2”和“热研88—13”获得113棵完整植株。并首次盆栽成活,成活率30％。到目前为止,已从“海垦2”、“海垦1”、“热研88—13”等品种中诱导出完整植株137棵,1979年移栽成活率提高到69％。经过几年的试验,得到了培养效果较好的下列培养基:用以诱导愈伤组织和幼小胚状体的第一培养基为MS基本培养基添加5％椰乳、0.5～1.0毫克/升2,4—D和0.5～1.0毫克/升K,蔗糖提高到7～10％,用以继续诱导肉眼可见胚状体的第二培养基为MS基本培养基添加0.1～0.2毫克/升NAA,0.2～1.0毫克/升K和0～10毫克/升水解DNA,蔗糖提高到7％,微量元素可提高1～2倍;用以诱导植株的第三培养基为改良的MS培养基,其大量元素减为60～80％,微量元素加倍,蔗糖提高到5％,再添加2毫克/升GA。对非完整植株,再加0.5毫克/升K和0.5毫克/升BAP,有促进顶芽长出真叶,成为完整植株的作用。接种后45～50天是转移愈伤组织的最适时间。胚状体和植株的诱导频率因材料和花期的不同而有很大差异。经组织学和细胞学研究,证明花药植株起源于花药的体细胞。
Since the cultivation of Brazilian rubber anthers in 1973, callus with high induction frequency and nearly 40,000 embryoids have been produced. From the late 1977 to the spring of 1978, 113 complete plants were obtained from the high yielding clones, “Kennamen 2” and “Kenyan 88-13”. And for the first time potted survival, survival rate of 30%. So far, 137 complete plants have been induced from varieties such as “Hacon 2”, “Hailin 1” and “Heyan 88-13”. In 1979, the survival rate of transplanting increased to 69%. After several years of experimentation, the following media with better culture results were obtained: First medium used to induce callus and young embryoid bodies Added 5% coconut milk to MS basal medium, 0.5-1.0 mg / l 2,4-D and 0.5-1.0 mg / L K, and sucrose was raised to 7-10% for the second medium to continue inducing the macroscopic embryoid body to add 0.1-0.2 mg / L NAA to MS basal medium, 0.2 to 1.0 mg / L K and 0 to 10 mg / L hydrolysis of DNA, sucrose increased to 7%, trace elements can be increased 1 to 2 times; used to induce the third medium of the plant modified MS medium, the large The element is reduced to 60-80%, the trace element is doubled, the sucrose is increased to 5%, and then 2 mg / L GA is added. For non-intact plants, plus 0.5 mg / L K and 0.5 mg / L BAP, there is an effect of promoting the emergence of true leaves in the apical bud and becoming an intact plant. 45 to 50 days after inoculation is the optimal time to transfer callus. The frequency of embryoid body and plant induction varies widely depending on the material and flowering stage. Histological and cytological studies have shown that anther plants originate from anther somatic cells.