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目的:建立高效液相色谱法(HPLC)测定七宝美髯口服液中2,3,5,4-四羟基二苯乙烯-2-O-B-D-葡萄糖苷(以下简称为二苯乙烯苷)含量的方法。方法:采用Aglent Zorbax SB-C18(4.6mm×250mm,5μm)色谱柱,以乙腈-水(20∶80)为流动相;流速为1.0mL/min;检测波长为320nm;柱温为30℃。结果:2,3,5,4-四羟基二苯乙烯-2-O-B-D-葡萄糖苷对照品在9.7～77.6μg/mL范围内,与峰面积呈良好的线性关系,回归方程为Y=3.94×103 X+2.75×103,r=0.9997,平均回收率为98.03%,RSD为0.98。结论:该方法操作简单,准确,重现性好,可用于七宝美髯口服液中二苯乙烯苷的质量控制。
Objective: To establish a method for the determination of 2,3,5,4-tetrahydroxystilbene-2-O-B-D-glucoside (abbreviated as stilbene glycoside) in Qibao Meixi oral solution by high performance liquid chromatography (HPLC) Methods: Aglent Zorbax SB-C18 (4.6mm × 250mm, 5μm) was used with mobile phase of acetonitrile-water (20:80) as mobile phase at a flow rate of 1.0mL / min. The detection wavelength was 320nm. The column temperature was 30 ℃. Results: The calibration curve of 2,3,5,4-tetrahydroxystilbene-2-OBD-glucoside was in the range of 9.7 ~ 77.6μg / mL with a good linear relationship with the peak area. The regression equation was Y = 3.94 × 103 X + 2.75 × 103, r = 0.9997, the average recovery was 98.03%, RSD was 0.98. Conclusion: The method is simple, accurate and reproducible. It can be used for the quality control of stilbene glycosides in Qibao Meixi oral solution.