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[目的]了解2012年上海部分0～5周岁儿童的血清轮状病毒LLR型中和抗体水平及不同年龄段儿童轮状病毒感染的情况,为疫苗的研究提供科学数据。[方法]在2012年6—8月上海市静安区儿保门诊部就诊的儿童中,筛选无发热、无轮状病毒感染疑似症状的健康儿童血清标本210份,采用微量中和试验方法检测儿童血清对轮状病毒LLR型疫苗株的中和抗体水平,并对检测结果进行统计学分析。[结果]210份0～5周岁儿童血清中,轮状病毒LLR型中和抗体阳性率为27.1%,中和抗体几何平均滴度(GMT)为1∶35.36;0～6个月婴儿血清中和抗体阳性率为16.0%,GMT为1∶28.72;7～11个月儿童血清中和抗体阳性率为2.5%,GMT为1∶25.88。1～2周岁儿童的中和抗体阳性率为20.0%,GMT为1∶30.78,2～3周岁儿童的中和抗体阳性率为20.0%,GMT为1∶32.99,该两年龄组(段)间差异无统计学意义。3～4周岁儿童的中和抗体阳性率为56.67%,GMT为1∶54.84,4～5周岁儿童的中和抗体阳性率为63.33%,GMT为1∶44.55,此两年龄组(段)儿童的阳性率和GMT均显著高于3周岁以下各年龄组,此两组间阳性率差异无统计学意义。[结论]0～3周岁儿童中和抗体水平较低,特别是7～11个月年龄组为最低,提示可能为轮状病毒感染的高危儿童群体。
[Objective] To understand the serum levels of rotavirus LLR neutralizing antibodies and rotavirus infection in children of different ages in Shanghai in 2012 from 0 to 5 years old, and to provide scientific data for the study of the vaccine. [Methods] From June to August 2012, 210 children with fever-free and non-rotavirus suspected symptoms in healthy children were screened from children who were treated by the Pediatrics Clinic in Jing’an District, Shanghai. Trace-neutralization test was used to detect children Serum levels of neutralizing antibodies against rotavirus LLR vaccine strains and statistical analysis of the test results. [Results] The positive rate of rotavirus LLR neutralizing antibody in sera from 210 children aged 0-5 years was 27.1%, and the geometric mean titer of neutralizing antibody (GMT) was 1:35.36. Serum samples from 0 to 6 months And the positive rate of antibody was 16.0% and the GMT was 1:28.72. The positive rate of serum neutralizing antibody in children aged 7-11 months was 2.5% and the GMT was 1:25.88. The positive rate of neutralizing antibody in children aged 1-2 years was 20.0% , The GMT was 1:30.78, the positive rate of neutralizing antibodies in children aged 2 ~ 3 years was 20.0% and the GMT was 1:32.99. There was no significant difference between the two age groups (section). The positive rate of neutralizing antibody in children aged 3 ~ 4 years was 56.67%, the GMT was 1:54.84, the positive rate of neutralizing antibody in children aged 4 ~ 5 years was 63.33%, the GMT was 1:44.55, the children of both age groups The positive rate and the GMT were significantly higher than those under 3 years of age, the positive rate of the two groups was no significant difference. [Conclusion] The level of neutralizing antibody in children aged 0 ~ 3 years old is low, especially in the 7 ~ 11 months age group, suggesting that it may be a high risk group of children infected with rotavirus.