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目的探讨新鲜血痕在不同直接扩增试剂盒的试验条件。方法存放2d内的新鲜血痕FTA卡540份和存放1~3m的陈旧性血痕FTA卡270份,各分别随机均分为3组,每份血痕打3片1.0mm纸片,分别用DNATyperTM15Plus试剂盒、GoldeyeTM20A试剂盒和华夏TM试剂盒在标准条件(说明书条件)、优化条件1(标准条件+1μL DMSO)和优化条件2(标准条件+室温浸泡1h)扩增检验,比较在3种条件下各组STR检验成功率。结果陈旧血痕样本用3种直扩试剂盒,在3种条件下,检验成功率均>97.00%,且均高于新鲜血痕。新鲜血痕在标准条件和优化条件1下,成功率为27.22%~31.67%,在优化条件2下,检验成功率相似(>97.00%),与标准条件和优化条件1比较,有统计学差异(P<0.01)。结论新鲜血痕在加入直接扩增试剂后于室温浸泡1h,可有效提高STR检验成功率。
Objective To investigate the experimental conditions of the new bloodstains in different direct amplification kits. Methods 540 fresh FTA cards and 270 old FTA cards stored 1 ~ 3m were stored within 2 days. Each of them was randomly divided into 3 groups. Each bloodstain was labeled with 1.0mm pieces of paper, and were respectively labeled with DNATyperTM15Plus kit , GoldeyeTM20A kit and Huaxia TM kit under the standard conditions (instruction conditions), optimization conditions 1 (standard conditions + 1μL DMSO) and optimization conditions 2 (standard conditions + room temperature soak 1h) amplification test, comparing the three conditions Group STR test success rate. Results The old bloodstains samples were tested with three kinds of DSS kits. The test success rates were> 97.00% under three conditions, and were higher than the fresh bloodstains. The success rate of fresh bloodstains was 27.22% ~ 31.67% under the standard conditions and optimization conditions 1, and the success rates were similar (> 97.00%) under the optimized conditions 2, which were statistically different from the standard conditions and optimization conditions 1 P <0.01). Conclusion The fresh bloodstains soaked in direct amplification reagent for 1h at room temperature can effectively improve the success rate of STR test.