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目的:探讨小鼠心脏骤停心肺复苏术后行为学和形态学的改变。方法:采用静脉注射氯化钾诱导心脏骤停的方法建立心脏骤停/心肺复苏(CA/CPR)小鼠模型。通过旷场实验观察小鼠行为学改变。制备脑片行HE染色,在光学显微镜及超微电镜下观察脑细胞形态的改变及细胞内超微机构的改变。结果:心肺复苏后野生型(WT:C3H/HeN)和突变型(TLR4-/-:C3H/HeJ)模型组小鼠周边区活动时间增加,中央区活动时间和路程显著减少,且WT模型组在中央区的活动时间较TLR4-/-模型组显著减少。光镜下发现TLR4-/-模型组脑细胞较WT模型组水肿、变性坏死较轻。超微电镜下发现海马及皮质脑细胞损伤最明显,线粒体、内质网和高尔基体等重要细胞器损伤,突触结构模糊,血管周围水肿。结论:从行为学方面来讲,心肺复苏后WT和TLR4-/-模型组小鼠趋避性及焦虑水平较高,且WT模型组较TLR4-/-模型组小鼠趋避性及焦虑水平更高。从形态学方面来讲,心肺复苏后TLR4-/-模型组脑细胞损伤较WT模型组轻。心脏骤停/心肺复苏后,TLR4-/-小鼠脑组织损伤程度均明显轻于WT小鼠,表明Toll样受体4在心肺复苏后脑细胞损伤中起重要作用。
Objective: To investigate the behavioral and morphological changes of cardiac arrest after cardiopulmonary resuscitation in mice. Methods: A cardiac arrest / cardiopulmonary resuscitation (CA / CPR) mouse model was established by intravenous injection of potassium chloride to induce cardiac arrest. Behavioral changes in mice were observed by open-field experiments. The brain slices were prepared and stained with HE. The morphological changes of brain cells and ultrastructural changes were observed under optical microscope and ultramicroscope. Results: After CPR, the peripheral time of mice in the wild type (WT: C3H / HeN) and mutant (TLR4 - / -: C3H / HeJ) model groups increased significantly and the time and distance traveled in the central area decreased significantly. The activity time in Central District was significantly lower than that in TLR4 - / - model group. Light microscope showed TLR4 - / - model group of brain cells than the WT model group edema, degeneration and necrosis lighter. The damage of hippocampus and cortex brain cells was found under the ultramicroscopic electron microscope. The damage of important organelles such as mitochondria, endoplasmic reticulum and Golgi apparatus, fuzzy synaptic structure and perivascular edema were observed. CONCLUSION: From behavioral aspects, the mice in WT and TLR4 - / - mice after cardiopulmonary resuscitation have higher levels of avoidance and anxiety, and WT and TLR4 - / - mice have higher avoidance and anxiety levels . Morphologically, the damage of brain cells in TLR4 - / - model group after cardiopulmonary resuscitation was lighter than WT model group. After cardiac arrest / cardiopulmonary resuscitation, brain injury in TLR4 - / - mice was significantly lighter than that in WT mice, indicating that Toll - like receptor 4 plays an important role in the injury of brain cells after cardiopulmonary resuscitation.