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【目的】探讨补阳还五汤对慢性脑缺血大鼠学习记忆能力的影响及其可能的作用机制。【方法】选用SPF级SD大鼠,采用双侧颈总动脉结扎法复制慢性脑缺血大鼠模型,再将经Morris水迷宫筛选的合格模型动物随机分为模型组,金纳多组(剂量为45 mg.kg-1.d-1),补阳还五汤高、低剂量组(中药高、低剂量组,剂量分别为40、20 g.kg-1.d-1),并与假手术组进行对照,检测各组大鼠的学习记忆能力及脑组织中血小板活化因子(PAF)含量。【结果】在Morris水迷宫定向航行实验中,给药各组第1天及中药低剂量组第2、3天逃避潜伏期明显缩短,与模型组比较差异均有显著性意义(P<0.05);在空间探索实验中,中药各组穿越平台次数均有增加,但与模型组比较差异无显著性意义(P>0.05);模型组脑组织PAF含量与假手术组比较显著升高(P<0.05),各给药组脑组织PAF含量显著降低(与模型组比较,P<0.05)。【结论】补阳还五汤能明显改善慢性脑缺血大鼠的学习记忆能力,其作用机制可能与降低脑组织中PAF含量有关。
【Objective】 To investigate the effect of Buyang Huanwu Decoction on learning and memory abilities in rats with chronic cerebral ischemia and its possible mechanism. 【Methods】 Sprague-Dawley rats were randomly divided into model group, Ginaton group (dose group), model group (45 mg.kg-1.d-1), Buyang Huanwu Decoction high and low dose group (high and low dose group, the dose was 40, 20 g.kg-1.d-1) Sham operation group were compared, learning and memory ability of rats and the content of platelet activating factor (PAF) in brain were detected. [Results] In the Morris water maze directed navigation experiments, the escape latency was significantly shortened on the 1st day of each group and on the 2nd and 3rd days of the low dose of traditional Chinese medicine group, with significant difference compared with the model group (P <0.05). In space exploration experiment, the number of each group of TCM crossed the platform increased, but there was no significant difference compared with the model group (P> 0.05); PAF content in model group was significantly higher than that in sham operation group (P <0.05) ). The PAF content in brain tissue of each administration group was significantly decreased (P <0.05 compared with model group). 【Conclusion】 Buyang Huanwu decoction can significantly improve the learning and memory abilities of rats with chronic cerebral ischemia, and its mechanism may be related to the decrease of PAF content in brain tissues.