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本文采用交替三线性分解(ATLD)和交替归一加权残差三线性分解(ANWE)两种二阶校正方法结合激发发射矩阵荧光光谱对完全不经任何预处理的细胞培养基中的阿霉素进行简单、快速、直接的定量测定.当算法选取组分数为2时,解析得到细胞培养基中阿霉素的平均回收率分别为(100.5±1.8)%和(100.3±1.9)%.在细胞培养基中加入烟酰胺腺嘌呤二核苷酸(NADH)、烟酰胺腺嘌呤二核苷酸磷酸(NADPH)、黄素腺嘌呤二核苷酸(FAD)和黄素单核苷酸(FMN)四种细胞内的自发荧光物后,选取组分数为4时,解析得到细胞培养基中阿霉素的平均回收率分别为(99.1±2.9)%和(99.2±3.1)%.结果表明该分析方法能够准确、快速地直接测定细胞培养基中阿霉素的含量,并且在模拟细胞内荧光干扰环境下可定量测定阿霉素,且能获得令人满意的结果.
In this paper, two kinds of second-order calibration methods, alternating trilinear decomposition (ATLD) and alternating normalized weighted residual trilinear decomposition (ANWE), were combined with excitation emission matrix fluorescence spectroscopy for doxorubicin in cell culture medium without any pretreatment (100.5 ± 1.8)% and (100.3 ± 1.9)%, respectively.The average recoveries of doxorubicin in cell culture medium were calculated by the method of selecting 2 fractions.The average recoveries of doxorubicin in cell culture medium were (100.5 ± 1.8)% and In the medium, nicotinamide adenine dinucleotide (NADH), nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) (99.1 ± 2.9)% and (99.2 ± 3.1)%, respectively, of the cell culture medium after the autofluorescent material was selected from the cells in the presence of 4 components.The results showed that the method The content of doxorubicin in the cell culture medium can be accurately and quickly determined, and doxorubicin can be quantitatively measured in the simulated intracellular fluorescence interference environment, and satisfactory results can be obtained.