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目的探讨安多霖对微波辐射致大鼠海马差异基因表达的影响。方法采用30 mW/cm2的微波辐射40只雄性SD大鼠,分为正常对照组、辐射对照组、安多霖预防组和治疗组,后两组分别于辐射前或辐射后给予12 g/(kg·d)的安多霖,每天1次,连续14 d。利用大鼠寡聚核苷酸芯片检测30 mW/cm2微波辐射后14 d辐射组和安多霖组的大鼠海马差异基因,并进行功能分类;通过RT-PCR方法验证部分基因表达;分析相关基因在微波辐射损伤中的作用。结果 30 mW/cm2微波辐射后14 d,大鼠海马组织中表达差异基因19个(上调12个,下调7个);预防性给予安多霖,差异表达基因4个(上调1个,下调3个);治疗性给予安多霖,差异表达基因10个(上调6个,下调4个)。RT-PCR验证结果与芯片结果一致。差异表达基因涉及能量代谢、递质运输等,安多霖药物组中促恢复的基因表达增强。结论 30 mW/cm2微波辐射使大鼠海马中Grid2、Alas2等基因差异表达,安多霖可通过对该基因表达的影响促进微波辐射脑损伤的恢复。
Objective To investigate the effect of Andorin on the differential gene expression in hippocampus induced by microwave radiation in rats. Methods Forty male Sprague-Dawley rats were irradiated with 30 mW / cm2 microwave and divided into normal control group, radiation control group, Andolimin prophylaxis group and treatment group. The latter two groups were given 12 g / ( kg · d) Anduo Lin, 1 day, for 14 days. The rat hippocampus differential genes were detected by rat oligonucleotide microarrays after radiation for 30 days at 30 mW / cm2 and groups treated with Andolimin for functional classification. The expression of some genes was verified by RT-PCR and the correlation was analyzed Gene in the role of microwave radiation injury. RESULTS: At 14 d after 30 mW / cm2 microwave irradiation, there were 19 differentially expressed genes in the hippocampus of rats (up-regulated by 12 and down-regulated by 7), and prophylactically treated with Andolin, 4 differentially expressed genes (one up-regulated, ); Therapeutic given Andorin, 10 differentially expressed genes (up 6, down 4). The result of RT-PCR is consistent with the result of the chip. Differentially expressed genes involved in energy metabolism, neurotransmitter transport, Andorin drug group to promote the recovery of gene expression increased. Conclusion Microwave radiation at 30 mW / cm2 can induce the differential expression of genes such as Grid2 and Alas2 in rat hippocampus. Andorolin can promote the recovery of brain injury induced by microwave radiation.