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目的研究肝癌细胞和白血病细胞诱导分化时α１，６岩藻糖转移酶（α１，６ＦｕｃＴ）的改变。方法采用生物素标记受体糖链，用ＬＣＡ亲和层析柱分离被核心岩藻糖化的产物，并以辣根过氧化酶（ＨＲＰ）标记的亲和素与柱上的产物结合，最后测定洗脱液中ＨＲＰ的活力，可代表α１，６ＦｕｃＴ活力。结果全反式维甲酸（ＡＴＲＡ）或双丁酰环磷腺苷（ｄｂ－ｃＡＭＰ）诱导７７２１人肝癌细胞分化后，α１，６－ＦｕｃＴ活力均降低。ＡＴＲＡ或正丁酸使ＨＬ－６０早幼粒白细胞分化成粒细胞，佛波醇－１２－豆蔻酸－１３乙酸酯（ＰＭＡ）使ＨＬ－６０细胞分化成单核细胞时都使α１，６ＦｕｃＴ活力下降，而用二甲亚砜（ＤＭＳＯ）或神经节苷酯ＧＭ３使ＨＬ－６０细胞分化成粒细胞或单核细胞时，α１，６ＦｕｃＴ活力则上升。结论α１，６－ＦｕｃＴ活力下降是肝癌细胞的分化指标，但和ＨＬ－６０细胞的分化关系不大。
Objective To investigate the alteration of α1,6 fucosyltransferase (α1,6FucT) in the differentiation of hepatoma cells and leukemia cells. Methods The biotin-labeled receptor sugar chain was used to separate the core fucosylated product by an LCA affinity chromatography column. The horseradish peroxidase (HRP)-labeled avidin was combined with the product on the column and finally determined. The activity of HRP in the eluent can represent α-1,6-FucT activity. Results All-trans retinoic acid (ATRA) or db-cAMP induced a decrease in the activity of α1,6-FucT after the differentiation of 7721 human hepatoma cells. ATRA or n-butyric acid differentiates HL-60 promyelocytic leukocytes into granulocytes, and phorbol-12-myristate-13 acetate (PMA) makes α -1 when differentiating HL-60 cells into mononuclear cells. The activity of 6-FucT decreased. When HL-60 cells were differentiated into granulocytes or mononuclear cells with dimethyl sulfoxide (DMSO) or GM3 ganglioside, the activity of α-1,6-FucT increased. Conclusion The decreased activity of α1,6-FucT is an indicator of differentiation of hepatocellular carcinoma cells, but has little relationship with the differentiation of HL-60 cells.