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目的分析线栓法大脑中动脉阻塞(MCAO)脑缺血—再灌注模型大鼠的行为学特点,及其与梗死体积的关系。方法选择成年健康雄性SD大鼠108只,通过线栓法制作大脑中动脉阻塞后脑缺血—再灌注模型,阻塞100 min后拔除线栓再灌注,MCAO术后24 h行核磁共振扫描明确梗死部位并计算梗死灶体积,依据是否存在梗死灶及梗死灶部位将大鼠分为:皮质及基底节梗死组(CBG组,n=53)、基底节梗死组(BG组,n=29)及无梗死组(N1组,n=26)。应用Bederson评分,横木行走实验及足失误测试对大鼠MCAO术后24h的神经功能损害程度进行评价,并分析3组大鼠行为学评分及其与梗死体积的相关性。结果 NI组、BG组的Bederson评分明显低于CBG组(q=4.56、4.32,P均<0.01),而BG组及NI组评分差异无统计学意义(q=0.42,P=0.897);NI组、BG组的横木行走实验评分明显高CBG组(q=4.22、3.98,P<0.01),而BG组及NI组评分差异无统计学意义(q=0.24,P=0.101);NI组、BG组的足失误率明显低于CBG组(q=3.68、3.24,P<0.05),而BG组及NI组足失误率差异无统计学意义(q=0.46,P=0.830)、CBG组脑梗死体积明显大于BG组脑梗死体积,差异有统计学意义[(276.05±90.11)mm~3 vs.(60.03±25.17)mm~3,t=15.856,P<0.01],大鼠Bederson评分、横木行走实验及足失误测试中的行为学评分与其梗死体积之间均无线性相关性(CBG组:r=-0.149、-0.231、-0.186,P=0.308、0.111、0.249;BG组:r=0.187、-0.332、0.291,P=0.381、0.113、0.168)。结论通过线栓法制作大鼠大脑中动脉阻塞脑缺血-再灌注模型可出现2种行为学表现差异显著的梗死类型,且各个类型的行为学评分与其梗死体积大小不呈正比。
Objective To analyze the behavioral characteristics of MCAO cerebral ischemia - reperfusion model rats and its relationship with infarct volume. Methods One hundred and eight adult male Sprague-Dawley rats were randomly divided into three groups: the middle cerebral artery occlusion (MCAO) cerebral ischemia-reperfusion model was established by thread occlusion; the reperfusion was removed after occlusion for 100 min; the infarct was identified 24 h after MCAO The volume of infarct volume was calculated. According to the presence or absence of infarction and infarct site, rats were divided into three groups: cortical and basal ganglia infarction group (CBG group, n = 53), basal ganglia infarction group (BG group, n = 29) Infarcted group (N1 group, n = 26). The Bederson score, crossbar walking test and foot failure test were used to evaluate the degree of neurological damage 24h after MCAO in rats. The behavioral score and its correlation with infarct volume were analyzed. Results The Bederson score of NI group and BG group was significantly lower than that of CBG group (q = 4.56, 4.32, P <0.01), but there was no significant difference between BG group and NI group (q = 0.42, P = 0.897) In the NI group, there was no significant difference between BG group and NI group (q = 0.24, P = 0.101) The foot failure rate in BG group was significantly lower than that in CBG group (q = 3.68,3.24, P <0.05), while there was no significant difference in foot failure rate between BG group and NI group (q = 0.46, P = 0.830) The volume of infarction was significantly larger than that of BG group ([(276.05 ± 90.11) mm ~ 3 vs. (60.03 ± 25.17) mm ~ 3, t = 15.856, P <0.01] There was no linear correlation between behavioral score and infarct volume in walking test and foot failure test (CBG group: r = -0.149, -0.231, -0.186, P = 0.308,0.111,0.249; BG group: r = 0.187 , -0.332, 0.291, P = 0.381, 0.113, 0.168). Conclusions There are two kinds of infarction types with significant difference in behavioral performance in middle cerebral artery occlusion model of cerebral ischemia-reperfusion induced by thread plug method, and each type of behavioral score is not proportional to the infarct size.