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目的:建立溃疡性结肠炎(UC)大鼠模型,研究溃克灵(KKL)对大鼠溃疡性结肠炎的治疗作用及对血清白细胞介素-8(IL-8)产生和表达的影响,探讨KKL防治溃疡性结肠炎的作用机制。方法:采用2,4,6一三硝基苯磺酸(TNBS)法(100mg/kg TNBS+50%的乙醇0.25ml)灌肠复制出溃疡性结肠炎大鼠模型。将72只大鼠随机分为正常组、模型组、柳氮磺吡啶(SASP)组、KKL大、中、小剂量组,观察结肠大体形态损伤、组织学变化,ELISA法测定各组大鼠血清IL-8水平。结果:与模型组相比,KKL各组、SASP组结肠大体形态损伤、组织学变化显著改善,血清IL-8水平显著降低(P<0.01)。结论:溃克灵对大鼠UC有显著的治疗作用,抑制促炎因子IL-8的产生和表达可能是其治疗机制之一。
OBJECTIVE: To establish a rat model of ulcerative colitis (UC) and to study the therapeutic effect of croconazole (KKL) on ulcerative colitis in rats and its effect on the production and expression of serum interleukin-8 (IL-8). To explore the mechanism of action of KKL in the treatment of ulcerative colitis. METHODS: The rat model of ulcerative colitis was reproduced by enema with 2,4,6-trinitrobenzene sulfonic acid (TNBS) (100 mg/kg TNBS + 50% ethanol 0.25 ml). 72 rats were randomly divided into normal group, model group, sulfasalazine (SASP) group, and KKL large, medium and small dose groups. The gross morphology damage and histological changes of the colon were observed. The serum of each group was determined by ELISA. IL-8 levels. RESULTS: Compared with the model group, the gross morphology and histological changes of the colon of the KKL and SASP groups were significantly improved, and serum IL-8 levels were significantly decreased (P<0.01). Conclusion: Kuikeling has a significant therapeutic effect on UC in rats. The inhibition of the production and expression of proinflammatory cytokines IL-8 may be one of its therapeutic mechanisms.