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目的:建立测定添加在降糖中成药中的格列吡嗪、格列本脲、格列齐特、格列喹酮、格列美脲、盐酸吡格列酮、盐酸罗格列酮、那格列奈、瑞格列奈共9种化学降糖药的高效液相分析方法。方法:色谱柱:Thermo C_(18)(150 mm×4.6 mm,5μm),流动相:醋酸盐缓冲溶液(10 mmol·L~(-1)醋酸铵和20 mmol·L~(-1)三乙胺,醋酸调pH至5.5)-乙腈(61:39),流速:1 ml·min~(-1),检测波长:210 nm,柱温:30℃,进样20μl。结果:9种化学降糖药完全分离,检出限为4～18 ng;9种药物具有宽的线性范围和良好的线性关系(r≥0.999 4);日内、日间RSD分别为0.09%～1.42%和0.30%～1.88%;在中药中的加样回收率除个别药物低浓度外均大于80%,RSD为0.26%～5.91%。结论:该方法用于检测中成药中的化学降糖药快速、简便、高效。
Objective: To establish a method for the determination of glipizide, glibenclamide, gliclazide, gliquidone, glimepiride, pioglitazone hydrochloride, rosiglitazone hydrochloride, nateglinide , Repaglinide a total of nine kinds of chemical hypoglycemic agents HPLC method. METHODS: The mobile phase consisted of acetate buffer solution (10 mmol·L -1 ammonium acetate and 20 mmol·L -1) of Thermo C 18 (150 mm × 4.6 mm, 5 μm) Triethylamine and acetic acid were adjusted to pH 5.5) -acetonitrile (61:39). The flow rate was 1 ml · min -1. The detection wavelength was 210 nm and the column temperature was 30 ℃. Results: Nine kinds of chemical hypoglycemic agents were completely separated with a detection limit of 4-18 ng. Nine kinds of drugs had a wide linear range and a good linear relationship (r≥0.999 4). The intra-day and inter-day RSD were 0.09% 1.42% and 0.30% ~ 1.88% respectively. The recoveries of samples in Chinese medicine were all higher than 80% and the RSD was 0.26% ~ 5.91% except for the low concentration of some drugs. Conclusion: The method is rapid, simple and effective for the detection of chemical hypoglycemic agents in proprietary Chinese medicines.