论文部分内容阅读
紫外分光光度法测定水扬酸搽剂的含量山西省１０９医院（０３０００６）邱风婷，王亚利哈医大一院宋永熙根据水杨峻在紫外２９６±１ｎｍ处有最大吸收特点，采用紫外分光光度法测定水扬酸搽剂的含量，并与滴定法相比，表明紫外法具有方法简便、快速、回收率好的特点。材料与方法一、材料：ＵＶ一２６５紫外分光光度计，日本岛津产；水杨酸原料（批号９１０８０７）山东新华制药厂产；水杨酸搽刑（批号９２０１２６），本院制剂室。二、方法：１。紫外吸收图谱的扫描：精取水扬酸搽剂５ｍｌ稀释至１００ｍｌ，然后分别取该稀释液１、２，３ｍｌ置１００ｍｌ容量瓶中，加蒸镏水稀释至刻度。于ＵＶ—２６５上波长２００～３５０ｍｍ内进行紫外图谱的扫描，结果在２９６ｎｍ处有最大吸收峰，因此本文选择在２９６±１ｎｍ。为最大吸收波长，来测定水扬酸的含量。２．标准曲线的制做：精密称取至恒重的水扬酸原料３２．１ｍｇ，置于１００ｍｌ容量瓶中，并加乙醇至刻度，精取１、２、３、４、５ｍｌ分别置于１００ｍｌ容量瓶中加水至刻度，于ＵＶ一２６５上波长２９６ｍｍ处测定吸收度，得到浓度和吸收度的方程如下：ｃｐｇ／ｍｌ＝３９，０４５Ａ—０．９０６８ｒ＝０．９９９８３，加料回收率：于５％水杨
Determination of salicylic acid liniment by ultraviolet spectrophotometry Shanxi Provincial Hospital (030006) Qiu Fengting, Wang Yali Song Yongxi, a large hospital based on the water Shuang Jun Jun UV at 296 ± 1nm maximum absorption characteristics of UV spectrophotometry water Yang Acid liniment content, and compared with the titration method, indicating that UV method has the advantages of simple, rapid, good recovery characteristics. Materials and Methods First, the material: UV a 265 UV spectrophotometer, Shimadzu Japan; salicylic acid raw materials (batch number 910807) Shandong Xinhua Pharmaceutical Factory production; salicylic acid Wan punishment (lot number 920126), the preparation room. Second, methods: 1. Ultraviolet absorption spectrum of the scan: Sediment Sediment liniment 5ml diluted to 100ml, and then were taken to the diluent 1,2,3ml 100ml volumetric flask, add distilled water to the mark. UV-265 on the wavelength of 200 ~ 350mm within the UV spectrum of the scan, the result at 296nm at the maximum absorption peak, so the choice of this paper at 296 ± 1nm. For the maximum absorption wavelength, to determine the salicylic acid content. 2. Preparation of the standard curve: accurately weighed to the constant weight of salicylic acid raw material 32.1mg, placed in a 100ml volumetric flask, and add ethanol to the mark, fine take 1,2,3,4,5 ml were placed in 100ml capacity The flask was filled with water to the mark, and the absorbance was measured at a wavelength of 296 mm on a UV-265 to obtain the concentration and the absorbance as follows: cpg / ml = 39,045A-0.9068r = 0.99983, Salisbury