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目的分析中国不同人群及不同胃部疾病病例来源的幽门螺杆菌致病相关基因cagA、iceA、vacA及HP0519的分布。方法采用特异引物聚合酶链式反应(PCR)方法分析150株幽门螺杆菌上述基因的多态性分布特点,并对其分布作初步统计分析。结果93％(139／150)中国菌株cagA基因3′端重复序列的PCR产物具有东方菌株特征。75％(113／150)菌株iceA基因为iceA1, 19％(29／150)为iceA2,不同地区间iceA基因的分布差异无统计学意义。云南菌株iceA1、iceA2的分布与菌株分离个体的种族特点及临床疾病类型无显著关系。96％中国菌株(144／150)vacA基因s区的等位基因为s1;m区等位基因m2、m1b和m1b-m2的比例分别为57％(85／150)、27％(41／150)和11％(16／150),仅2株福建菌株为m1a。不同地区间vacA s1、m2、m1b分布的差异无统计学意义。云南菌株m1b-m2的分布高于福建和北京菌株。云南菌株vacA s区等位基因的多样性与分离个体的种族及临床疾病类型无显著关系。vacA m区等位基因的多样性与分离个体的临床疾病类型无显著关系,但不同民族间m2的分布有显著差异,白族人群m2的分布显著少于汉族和纳西族。93％(140／150)的中国菌株HP0519基因具有24 bp和15 bpDNA插入和缺失的多态性特点。不同地区间HP0519基因的多态性无显著不同。云南菌株HP0519的多态性与菌株分离个体的临床疾病类型无显著关系,但来源不同民族菌株的HP0519基因存在差异。结论幽门螺杆菌中国菌株cagA 3′端JF/TR特异引物的扩增结果具有东亚菌株特点。中国菌株vacA基因多为s1,其分布与菌株分离个体的临床疾病类型无关。中国菌株mcA基因m区的分布具有多样性。中国菌株HP0519基因具有24 bp和15 bp插入和缺失的多态性特点。
Objective To analyze the distribution of pathogenic genes cagA, iceA, vacA and HP0519 in Helicobacter pylori from different population and different gastric diseases in China. Methods The polymorphism distribution of the 150 H. pylori isolates was analyzed by polymerase chain reaction (PCR) and their distributions were analyzed statistically. Results 93% (139/150) of the PCR products of the 3 ’end of the cagA gene in Chinese strains had the characteristics of oriental strains. IceA gene of 75% (113/150) strain was iceA1, and 19% (29/150) was iceA2. There was no significant difference in the distribution of iceA gene among different regions. The distribution of iceA1 and iceA2 strains in Yunnan showed no significant relationship with the race characteristics and type of clinical disease in isolated strains. 96% of Chinese isolates (144/150) had s1 in the s region of the vacA gene and 57% (85/150), 27% (41/150) in the m-region alleles of m2, m1b and m1b- ) And 11% (16/150) respectively. Only two Fujian strains were m1a. There was no significant difference in the distribution of vacA s1, m2 and m1b between different regions. The distribution of m1b-m2 in Yunnan was higher than that in Fujian and Beijing. The diversity of vacA s allele in Yunnan isolates was not significantly related to the ethnicity and type of clinical disease in isolated individuals. There was no significant relationship between the diversity of vacA m allele and the type of clinical disease in isolated individuals, but the distribution of m2 among different ethnic groups was significantly different. The distribution of m2 in Bai ethnic group was significantly less than that of Han and Naxi ethnic groups. The 93% (140/150) Chinese isolate HP0519 gene has the characteristics of 24 bp and 15 bp DNA insertions and deletions. There was no significant difference in the polymorphism of HP0519 between different regions. The polymorphism of HP0519 in Yunnan was not related to the type of clinical disease in isolated strains, but there were differences in the HP0519 gene from different ethnic strains. Conclusion The amplified results of JF / TR specific primer of cagA 3 ’end of Helicobacter pylori in China have the characteristics of East Asian strains. Chinese strains vacA gene mostly s1, the distribution of strains isolated strains of clinical disease has nothing to do. The distribution of mcA gene m region in China is diversified. The Chinese isolate HP0519 has the characteristics of 24 bp and 15 bp insertions and deletions.