紫云英根瘤菌天然抗药性和质粒研究

来源 :华中农业大学学报 | 被引量 : 0次 | 上传用户:loughtjiang
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
从同一稻田的紫云英根瘤上共分离到154株紫云英根瘤菌。将来自该田块10株紫云英的100株根瘤菌列为分析组A;来自同一植株不同根瘤的40株根瘤菌列为分析组B;来自植株两个根瘤的14株根瘤菌列为分析组C。对所有菌株10种抗生素的抗药性测定表明,分析组A分为39个不同抗药类群;分析组B分为22个抗药类群。同一根瘤的分离株其抗药性也存在差异。质粒检测显示所有菌株都含有质粒,质粒数为1~5条;质粒分子量主要在83MD至600MD。质粒图谱分析表明,分析组A分为8个质粒型,分析组B分为6个质粒型,同一根瘤分离株的质粒数并不完全一致。在所有分离株中,第2质粒型的菌株为优势菌群。 A total of 154 strains of Astragalus sinicus were isolated from root nodules of the same rice field. The 100 Rhizobium strains from 10 Astragalus adsurgens of the field were classified as analysis group A. Forty Rhizobia strains from different root nodules of the same plant were classified as analysis group B. The 14 Rhizobium strains from two plant nodules were classified as analysis Group C. Resistance analysis of 10 antibiotics to all strains showed that analysis group A was divided into 39 different drug resistance groups; analysis group B was divided into 22 drug resistance groups. Isolates of the same nodule also have differences in drug resistance. Plasmid detection showed that all the strains contained plasmids, the number of plasmids was 1 to 5; the molecular weight of the plasmids mainly ranged from 83MD to 600MD. Plasmid map analysis showed that analysis group A was divided into eight plasmid types, and analysis group B was divided into six plasmid types. The plasmid numbers of the same root nodule isolates were not exactly the same. Among all the isolates, the second plasmid type strain was the dominant strain.
其他文献
目的:关于桡骨远端伸直型骨折患者以中医正骨手法救治的临床疗效分析.方法:随机筛选本院2015年01月-2016年01月救治的75例桡骨远端伸直型骨折患者,按照治疗方法进行分组治疗
目的:探讨二甲双胍联合格列齐特缓释片在2型糖尿病治疗中的应用效果.方法:对我院2013年6月-2016年6月期间72例2型糖尿病患者的病历资料进行回顾性分析,并根据患者意愿分为对
目的:激光治疗分泌性中耳炎的疗效.方法:应用激光鼓膜打孔加外耳道内照射治疗分泌性中耳炎与传统的鼓膜穿刺抽液和鼓室内注射地塞米松,比较疗效.结果:治疗组65例80耳,治愈76
请下载后查看,本文暂不支持在线获取查看简介。 Please download to view, this article does not support online access to view profile.
期刊
目的:探讨间歇性低氧对脑缺血大鼠磷酸酰肌醇3激酶(PI3K)活性的影响.方法:60只雄性Wistar大鼠采用数字随机法随机分成假手术组(SO组)、单纯全脑缺血再灌注组(I/R组)、间歇性
目的:探讨仅用UVB光疗治疗寻常型银屑病的疗效.方法:采用德国沃曼UVB光疗仪,患者治疗60天后观察疗效uvb光疗后皮肤颜色变化,干燥程度.结果:德国沃曼UVB光疗8周后,患者无明显
卵母细胞或胚胎在体外培养时得不到母体抗氧化剂的保护,产生的氧自由基会对卵母细胞的成熟及胚胎的发育产生抑制作用,这是制约卵母细胞体外培养效果的因素之一。表没食子儿茶素
期刊
请下载后查看,本文暂不支持在线获取查看简介。 Please download to view, this article does not support online access to view profile.
期刊
在合肥自然条件下,连续6年(1987~1992)观察了7001S育性和主要特征特性,研究了繁殖制种等配套技术。结果表明:(1)7001S属早熟晚粳型,播始历期122(单季制种)~80(双季繁殖)天。(2)不育期不育性稳定〔花粉败率率(SP)99.05%~99.94%,自交结实率(SS)0.00%~0.13%〕