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目的研究胸腺肽(Ta1)对人胆囊癌细胞株(GBC-SD)侵袭能力的影响。方法培养GBC-SD,将其分为对照组、不同浓度的Ta1给药组(100、200、400μg/m1)。经细胞侵袭实验观察Ta1对GBC-SD细胞侵袭能力的影响;MMPs明胶酶谱分析检测细胞MMP-2、MMP-9的蛋白表达变化。结果与对照组比较,随着Ta1给药浓度的升高,各组癌细胞的侵袭能力呈浓度依赖性下降,各组间的差异有统计学意义(P<0.05)。Ta1不同浓度组胆囊癌细胞MMP-9蛋白表达较对照组明显下降,且随着Ta1浓度的升高,MMP-9蛋白表达逐渐下降。而Ta1对MMP-2蛋白的表达无明显影响。结论 Ta1对GBC-SD侵袭能力具有抑制作用,且随Ta1浓度的升高,GBC-SD侵袭能力逐渐下降,其机制之一可能是降低了胆囊癌细胞MMP-9的蛋白质表达。
Objective To study the effect of thymosin (Ta1) on the invasiveness of human gallbladder carcinoma cell line (GBC-SD). Methods GBC-SD was cultured and divided into control group and different concentrations of Ta1 administration group (100, 200, 400 μg / ml). The effect of Ta1 on the invasiveness of GBC-SD cells was observed by cell invasion assay. The changes of MMP-2 and MMP-9 protein expression were detected by MMPs gelatin zymography. Results Compared with the control group, the invasion ability of cancer cells in each group decreased in a concentration-dependent manner with the increase of Ta1 concentration. The difference between the groups was statistically significant (P <0.05). The expression of MMP-9 in gallbladder carcinoma cells with different concentration of Ta1 was significantly lower than that in control group, and the expression of MMP-9 decreased with the increase of Ta1 concentration. However, Ta1 had no significant effect on MMP-2 protein expression. Conclusion Ta1 can inhibit the invasiveness of GBC-SD, and the invasion ability of GBC-SD gradually decreases with the increase of Ta1 concentration. One of the mechanisms may be that the protein expression of MMP-9 in gallbladder carcinoma cells is decreased.