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一、WOX的结构1996年,M.Aldaz实验室在研究乳腺原位癌时发现近70%的原位癌于16q23.2~16q24.1区域发生杂合性缺失[1]。2000年Bednarek等首次成功克隆出此区域的基因,并将其命名为WWOX(WW domain-containing oxidoreductase,含WW结构域的氧化还原酶),又名FOR(fragile site FRA16D oxidoreductase,脆性位点FRA16D氧化还原酶)[2]。该基因跨越1.11 Mb,包含9个外显子及8个内含子。由于剪切形式的不同可形成7种不同的异构体,最常见的异构体形式是全长WWOX,其大小约1.2 kb,编码414个氨基酸形成的蛋白质,蛋白分子量为46.6 kD。
First, the structure of WOX In 1996, M.Aldaz laboratory in the study of breast carcinoma in situ found that nearly 70% of in situ carcinoma in 16q23.2 ~ 16q24.1 region heterozygous deletions [1]. In 2000, Bednarek et al. Successfully cloned the gene of this region for the first time and named it WWOX (WW domain-containing oxidoreductase), also known as FOR (fragile site FRA16D oxidoreductase, fragile site FRA16D oxidation Reductase) [2]. The gene spans 1.11 Mb, contains 9 exons and 8 introns. Seven different isoforms can be formed due to different shear forms. The most common isoform, WWOX, is approximately 1.2 kb in size and encodes a protein of 414 amino acids with a molecular weight of 46.6 kD.