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用反相高浓度离子对HPLC法,以柔红霉素为内标,建立大鼠血清及组织(脑、心、肾、肝、肺、小肠和脾脏)中阿霉素及其代谢产物阿霉素醇浓度的测定方法。本法简便,重现性好,可用于常规分析。血清样品提取:取1ml大鼠血清置15ml硅烷化玻璃离心管中,加50μl浓度为2或10μg/ml的盐酸柔红霉素30%甲醇-水溶液。振荡混匀,加3ml 乙腈,振荡30s 后,离心20min(4℃,3000 r/min)。上清液移
Using reversed-phase high-concentration ion-pair HPLC method and daunorubicin as internal standard, Adriamycin and its metabolites of Aspergillus fumigatus in serum and tissues (brain, heart, kidney, liver, lung, small intestine and spleen) Method for the determination of the concentration of the alcohol. This method is simple, reproducible and can be used for routine analysis. Serum Sample Extraction: Take 1 ml of rat serum in a 15 ml silanized glass centrifuge tube and add 50 μl of daunorubicin 30% methanol-water solution at a concentration of 2 or 10 μg / ml. Mix well by shaking, add 3ml of acetonitrile, shake for 30s and centrifuge for 20min (4 ℃, 3000 r / min). The supernatant was removed