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为研究ＨＰＶ１６转录调节蛋白ＹＹ１结合位点改变对病毒致癌性的影响，以ＨＰＶ１６野毒株质粒ｐ１２０３为基础，经多次克隆，将来自宫颈癌组织并带有缺损突变的ＬＣＲ重组到地ＨＰＶ１６基因组中。核酸序列分析证实，质粒ｐＤＶ３９０在第２个ＹＹ１位点上有一Ｇ→Ａ点穷变，质粒ｐＤＶ３２６和ｐＤＶ４０１分别带有１１５ｂｐ和１４３ｂｐ的缺失突变，从而涉及２和４个ＹＹ１结合位点，重组质粒其它核苷酸序列与ＨＰＶ１６标准序列一致。这些重组质粒的组建为进一步研究ＹＹ１蛋白对ＨＰＶ１６致癌基因表达的调控及ＨＰＶ１６致癌作用的影响打下基础。
In order to study the effect of HPV 16 transcriptional regulatory protein YY1 binding site on the carcinogenicity of the virus, HPV 16 wild-type plasmid p1203 was used as a basis to clone HPV16 genomic DNA from cervical cancer tissues with deletion mutation after multiple cloning in. Nucleotide sequence analysis confirmed that the plasmid pDV390 has a G → A point mutation at the second YY1 locus, and the deletion mutations of 115 bp and 143 bp of the plasmids pDV326 and pDV401, respectively, involving 2 and 4 YY1 binding sites. The recombinant plasmid The other nucleotide sequences are identical to the HPV16 standard sequence. The establishment of these recombinant plasmids laid the foundation for the further study on the regulation of HPV16 oncogene expression and the carcinogenesis of HPV16 by YY1 protein.