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为比较外周血T淋巴细胞亚群CD4不同测定方法的差别 ,以流式细胞术为定量手段 ,测定了猴外周血中三种不同方法处理后CD4的表达 .结果表明 :先标后溶法———先用异硫氰荧光素标记的单克隆抗体 (FITC CD4McAb)标记后 ,再加入红细胞溶解液溶掉红细胞的处理方法 ,结果基本等同于传统的淋巴细胞分离法 ,但样本用量仅为传统方法的 1/ 5 ,且操作简单 .激光共焦显微术的形态学研究也证实 :先标后溶法与淋巴细胞分离法相似 ,其细胞膜表面荧光标记清晰 ,优于先溶后标法
In order to compare the difference of CD4 assay between peripheral blood T lymphocyte subsets and CD4 (superscript +) T lymphocyte subsets in peripheral blood, flow cytometry (FCM) - first labeled with isothiocyanate fluorescein monoclonal antibody (FITC CD4McAb) labeled, and then added erythrocyte lysate dissolved red blood cells, the results are basically the same as the traditional lymphocyte isolation method, but the sample amount is only traditional Method of 1/5, and the operation is simple.Laser confocal microscopy Morphology studies also confirmed that: after the standard solution and lymphocyte separation method similar to the cell membrane surface fluorescence labeling clear, better than the first solution after the standard method