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目的观察地龙、乌梢蛇对系膜增生性肾炎(MsPGN)大鼠诱导型一氧化氮合酶(iN-OS)、内皮素-1(ET-1)表达的影响,探讨其治疗作用及机制。方法用改良慢性血清病法制备MsPGN大鼠模型,以地龙、乌梢蛇高低剂量灌胃,设正常对照组及模型组,治疗8周后测血白蛋白(ALB)、肌酐(Scr)、尿素氮(BUN),观察肾脏病理变化及iNOS、ET-1表达。结果与正常对照组比较,模型组24 h尿蛋白、Scr及BUN升高(P<0.01)而ALB降低(P<0.01);与模型组比较,各治疗组24 h尿蛋白、Scr及BUN降低(P<0.05)且ALB升高(P<0.01),降24 h尿蛋白和升ALB作用地龙高剂量组优于低剂量组(P<0.05)。iNOS、ET-1表达水平模型组高于正常对照组(P<0.01),各治疗组低于模型组(P<0.01)。结论地龙、乌梢蛇均能降低MsPGN大鼠蛋白尿,升高ALB,地龙高剂量优于低剂量,乌梢蛇高、低剂量差异无统计学意义(P>0.05),二药均可降低Scr、BUN,其机制可能与抑制iNOS、ET-1在肾组织表达有关。
Objective To observe the effect of dilong and black snake snake on the expression of iNOS and ET-1 in rats with mesangial proliferative glomerulonephritis (MsPGN) mechanism. Methods The rat model of MsPGN was established by modified chronic serodiagnosis. The rats in normal control group and model group were treated with high and low dose of earthworm and black snake. After 8 weeks’ treatment, serum albumin (ALB), creatinine (Scr) Blood urea nitrogen (BUN), pathological changes of kidney and the expression of iNOS and ET-1 were observed. Results Compared with the normal control group, 24 h urinary protein, Scr and BUN increased (P <0.01) and ALB decreased (P <0.01). Compared with the model group, 24 h urinary protein, Scr and BUN decreased (P <0.05), ALB increased (P <0.01), decreased 24 h urinary protein and uplifted ALB in high dose group compared with low dose group (P <0.05). The expression of iNOS and ET-1 in the model group were higher than those in the normal control group (P <0.01), and those in the treatment group were lower than those in the model group (P <0.01). CONCLUSION Dulong and black snake snake can reduce the proteinuria of MsPGN rats, increase ALB, the high dose of earthworm is better than low dose, the high and low dose of snakehead snake has no statistical significance (P> 0.05) Can reduce Scr, BUN, the mechanism may be related to inhibition of iNOS, ET-1 expression in the kidney.