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目的 研究可控性表达的 p16基因对前列腺癌细胞的生长抑制作用。 方法 通过可诱导的真核表达载体 pMDNA 3 p16将外源野生型 p16基因转染至该基因表达功能丧失的人前列腺癌细胞PC3中 ,使用ZnSO4 作为诱导物 ,观察 p16基因的可控表达及其对前列腺癌细胞生物学特性的影响。结果 转染了野生型p16基因的人膀胱癌细胞PC3 pMDNA3 p16经ZnSO4 诱导后 ,开放了 p16基因的转录和翻译 ,p16蛋白得到高水平表达 ,细胞生长速度及集落形成率均有了部分抑制 ,细胞周期分析可见G1期增加 ,S期下降。结论 p16基因与前列腺癌的发生、发展有关 ,为用p16基因进行前列腺癌的基因治疗提供了实验依据。
Objective To study the inhibitory effect of p16 gene on the growth of prostate cancer cells. Methods The wild-type p16 gene was transfected into human prostate cancer cell line PC3 with loss of function by inducible eukaryotic expression vector pMDNA 3 p16. ZnSO4 was used as an inducer to observe the controllable expression of p16 gene and its On the biological characteristics of prostate cancer cells. Results The p16 gene transcripts and translations of human bladder cancer cell line PC3 transfected with wild-type p16 gene were induced by ZnSO4. The p16 protein was highly expressed and the cell growth rate and colony formation rate were partially inhibited. Cell cycle analysis shows increased G1 phase, S phase decreased. Conclusion The p16 gene is associated with the occurrence and development of prostate cancer and provides an experimental basis for the gene therapy of prostate cancer with p16 gene.