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选择健康的翘嘴鲌(Culter alburnus)为试验鱼,以红鱼粉为蛋白源,配制5个蛋白水平的等能、等必需氨基酸(EAA)平衡关联度的半精制饲料,又以豆粕替代鱼粉,大豆蛋白分别替代不同水平的鱼粉蛋白,配制5个EAA关联度的等蛋白、等能的半精制饲料,探讨饲料蛋白对鱼类生长和内分泌激素等的影响。结果表明:饲料蛋白水平对翘嘴鲌的特定增重率(SGR)具有显著影响(p<0.05),40.89%饲料蛋白组的SGR显著高于31.04%、35.51%饲料蛋白组(p<0.05),但与46.62%和50.33%饲料蛋白组没有显著性差异(p>0.05)。血清T3水平与饲料蛋白水平正相关(p<0.05)。血清GH水平与饲料蛋白水平和生长负相关(p<0.05),血清IGF-I水平与饲料蛋白水平和生长正相关(p<0.05)。适宜蛋白水平通过提高翘嘴鲌血清T3和IGF-I的水平来调控生长。当大豆蛋白分别替代54.0%的鱼粉蛋白时,翘嘴鲌的SGR与对照组差异显著(p<0.05)。翘嘴鲌血清T3与饲料大豆蛋白对鱼粉蛋白的替代量负相关(p<0.05)。40.5%和54.0%替代组的血清GH显著高于对照组(p<0.05),且与饲料中豆粕对鱼粉替代水平正相关(p<0.01),与生长负相关(p<0.05)。血清IGF-I与饲料中大豆蛋白对鱼粉蛋白替代量负相关(p<0.05),与生长正相关(p<0.05)。大豆蛋白的替代亦通过对内分泌激素的影响来调控生长。
Culter alburnus was selected as test fish and red fish meal was used as a protein source to prepare semi-refined forage with 5 levels of isoproterenol and other essential amino acids (EAA), and soybean meal was used instead of fishmeal. Soy protein instead of different levels of fish meal protein, preparation of five EAA degree of equal protein, isobaric semi-refined feed, to explore the impact of feed protein on fish growth and endocrine hormones. The results showed that the dietary protein level had a significant effect on SGR (p <0.05), SGR of 40.89% feed protein group was significantly higher than that of 31.04% and 35.51% feed protein group (p <0.05) , But no significant difference with 46.62% and 50.33% feed protein group (p> 0.05). Serum T3 levels were positively correlated with feed protein levels (p <0.05). Serum GH levels were negatively correlated with feed protein levels and growth (p <0.05), and serum IGF-I levels were positively correlated with feed protein levels and growth (p <0.05). Optimum protein levels control growth by elevating the levels of T3 and IGF-I in Culter alburnus. When soybean protein replaced 54.0% of the fish meal protein, the SGR of Culter alburnus was significantly different from that of the control group (p <0.05). Calycchial horn serum T3 was negatively correlated with dietary soy protein (p <0.05) on the replacement of fishmeal protein. Serum GH levels in 40.5% and 54.0% substitution groups were significantly higher than those in control group (p <0.05), and were positively correlated with dietary soybean meal (p <0.01) and negatively correlated with growth (p <0.05). Serum IGF-I was negatively correlated with dietary soy protein (p <0.05) for fish meal protein replacement, and positively correlated with growth (p <0.05). Substitution of soy protein also regulates growth by the effects on endocrine hormones.