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为考查温度对鱼类静止代谢率及低氧耐受能力的影响,本研究以中华倒刺鲃(Spinibarbus sinensis)幼鱼为对象,分别在10、20和30℃条件下驯化2周,随后将实验鱼置于密闭呼吸室中测定静止代谢率,并通过“双线法”推出临界氧压(Pcrit)等相关参数。实验结果显示:随着温度的上升,中华倒刺鲃幼鱼静止代谢率(MO2rest)、Pcrit点所对应代谢率(MO2pcrit)以及临界代谢范围(MMS)均显著上升(p<0.05);MO2rest的温度系数(Q10)在10~20℃和20~30℃间分别为2.54和1.27。而中华倒刺鲃幼鱼Pcrit却不受温度变化的显著影响,表现出一定的保守性。研究表明,中华倒刺鲃幼鱼Pcrit随温度的变化趋势并不与MO2rest的变化趋势相吻合,这提示随温度的改变中华倒刺鲃可能还启用了其他生理、生化和(或)行为学机制。
In order to investigate the effects of temperature on the resting metabolic rate and hypoxia tolerance of fish, Spinibarbus sinensis juveniles were acclimated for two weeks at 10, 20 and 30 ℃, respectively. The experimental fish were placed in a closed breathing chamber to measure the resting metabolic rate and the relevant parameters such as Pcrit were introduced by the “double line method”. The experimental results showed that as the temperature increased, the resting metabolic rate (MO2rest), the corresponding metabolic rate (MO2pcrit) and the critical metabolic range (MMS) of C. barbadense were significantly increased (p <0.05) The temperature coefficient (Q10) was 2.54 and 1.27 at 10 to 20 ° C and 20 to 30 ° C, respectively. However, Pcrit of Chinese barbed spiny juvenile fish did not significantly affect the temperature change, showing a certain degree of conservation. The results showed that the trend of Pcrit with temperature did not coincide with the trend of MO2rest, suggesting that other physiological, biochemical and / or behavioral mechanisms may also be activated with changes in temperature .