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目的 :比较黄山药总皂苷 (TSDP)和薯蓣皂苷元 (Dio)抗高脂血症及对胆固醇吸收的抑制作用。方法 :在形成高胆固醇动物模型同时或之后ig或ip给予TSDP和Dio ,以血清总胆固醇含量评价TSDP和Dio对高脂血症的预防和治疗作用。将TSDP或Dio与胆固醇及离体猪胆汁温孵形成微胶粒 ,以未形成微胶粒的游离胆固醇含量评价TSDP与Dio抑制胆固醇吸收作用。结果 :给小鼠igTSDP和Dio ,Dio对高脂血症有明显预防和治疗作用 ,而TSDP只有大剂量才有一定预防作用。小鼠ip给予Dio仍然有效 ,TSDP无效。大鼠igDio的预防效果明显优于TSDP。Dio体外抑制胆固醇微胶粒形成作用明显强于TSDP。结论 :Dio抗高脂血症作用优于TSDP ,可能与其抑制胆固醇微胶粒形成和吸收抑制有关系。
Objective : To compare the anti-hyperlipidemia and inhibition of cholesterol absorption of total saponin (TSDP) and diosgenin (Dio). METHODS: TSDP and Dio were given ig or ip at the same time or after the formation of a high-cholesterol animal model. The serum total cholesterol levels were used to evaluate the preventive and therapeutic effects of TSDP and Dio on hyperlipidemia. The TSDP or Dio was incubated with cholesterol and ex vivo porcine bile to form micelles. The free cholesterol content without micelles was used to evaluate the inhibition of cholesterol absorption by TSDP and Dio. RESULTS: Given that igTSDP and Dio were given to mice, Dio had significant preventive and therapeutic effects on hyperlipidemia, whereas TSDP only had a large dose to prevent it. Mouse ip administration to Dio was still effective and TSDP was ineffective. The preventive effect of igDio in rats was significantly better than TSDP. Dio inhibited the formation of cholesterol micelles significantly stronger than TSDP in vitro. Conclusion : Dio is superior to TSDP in antihyperlipidemia and may be related to the inhibition of cholesterol micelle formation and absorption inhibition.