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对泡沫分离nisin发酵液进行了研究,开发了两种由截流板和导流筒组成的促进泡沫排液的设备.通过实验验证了这两种设备的分离效果.实验结果表明第二种设备能够非常显著地降低出口持液率εout,提高出口泡沫的富集比.随着安装高度从0上升到15cm,εout从7.07‰下降到了6.13‰,泡沫液中nisin的最大效价可达39.6IU/μL.Nisin失活率稍有加重表明了这种方法在回收和浓缩蛋白质方面的应用潜力.最后,应用近年来的泡沫理论研究成果对设备的机理进行了解释.这一研究为日后多级排液泡沫塔的设计提供了基础,而多级排液泡沫塔有可能成为一种非常高效的分离设备。
The research on foam separation of nisin fermentation broth has been carried out and two kinds of devices to promote the foam drainage by the closure plate and the draft tube have been developed.The experimental results show that the second device can Very significantly reduce the holding rate εout of the outlet and increase the enrichment ratio of the outlet foam.As the installation height increases from 0 to 15cm, the εout decreases from 7.07 ‰ to 6.13 ‰, the maximum titer of nisin in the foam liquid can reach 39.6 IU / μL.Nisin inactivation rate slightly aggravated indicates the potential of this method in the recovery and concentration of protein.Finally, the application of foam theory in recent years, the research results on the mechanism of the device was explained.This study for the future multi-level row The design of the liquid bubble column provides the basis for which the multiple liquid bubble column may be a very efficient separation device.