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采用体外细胞培养和MTT测定等方法,研究羟基磷灰石微晶体(HAP—Sol)对人白血病细胞株(HL—60)的生长抑制作用。将不同浓度的HAP—Sol(0.001～0.02mg/ml)分别加入 HL—60细胞(10~4/孔),置 37℃,5％CO_2培养 48小时。结果显示:①不同浓度HAP—Sol对HL—60细胞集落形成可产生影响:在倒置显微镜下,可见全部测定孔细胞集落形成均较对照孔低下,且呈剂量依赖关系,同时出现萎缩和死亡细胞;②不同浓度HAP—Sol对HL—60细胞生长抑制率呈明显正相关性,在浓度为1.0,5.0,10.0.15.0,20.0时,抑制率相应为16.8,39.2,53.6,64.2,75.0。本研究进一步证实,HAP—Sol本身能明显抑制HL—60细胞生长,可能具有细胞毒作用。对白血病及肿瘤的治疗可能具有良好的开发前景。
In vitro cell culture and MTT assay methods to study the hydroxyapatite microcrystal (HAP-Sol) on human leukemia cell line (HL-60) growth inhibition. Different concentrations of HAP-Sol (0.001-0.02 mg / ml) were added to HL-60 cells (10-4 / well) and incubated for 48 hours at 37 ° C in 5% CO 2. The results showed that: (1) HAP-Sol with different concentrations had an effect on the colony formation of HL-60 cells: Under inverted microscope, all the measured colony formation of colony cells was lower than that of the control wells in a dose-dependent manner, with atrophy and dead cells ; ② HAP-Sol at different concentrations showed a significant positive correlation with the growth inhibition rate of HL-60 cells. At the concentrations of 1.0, 5.0, 10.0, 15.0 and 20.0, the inhibition rates were 16.8, 39.2, 53.6, 64.2 and 75.0, respectively. This study further confirmed that HAP-Sol itself can significantly inhibit the growth of HL-60 cells may have cytotoxic effects. The treatment of leukemia and cancer may have good development prospects.