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目的:探讨麝香通心滴丸对大鼠血管紧张素Ⅱ(AngⅡ)所致的内皮损伤是否有保护作用。方法:SD大鼠125只,分成1、3、7、14和28d组,每组25只,又分为假手术组5只,内皮损伤组和麝香组各10只。对照组埋置0.9%氯化钠液缓释泵;内皮损伤组埋置AngⅡ缓释泵,剂量为200ng·min-1·kg-1,麝香组在埋置AngⅡ缓释泵前3d至实验结束用麝香通心滴丸超细粉灌胃(剂量5mg/kg/d)。动物分别在术后1、3、7、14和28d处死,用放免法测定血液内皮素(ET)、一氧化氮(NO)、免疫比浊法测定C反应蛋白(CRP);ELISA测肿瘤坏死因子-α(TNF-α)含量。扫描电镜观察主动脉内皮形态的变化、透射电镜观察肾动脉内皮的结构。结果:假手术组内皮完好;内皮损伤组见内皮细胞(EC)充血、水肿、脱落,内弹力板暴露;而麝香组在1d组、3d组、7d组损伤相对较轻。14d组和28d组见EC有不同程度的恢复。结论:麝香通心滴丸可以降低血液中ET,CRP和TNF-α含量,增加NO的含量,对EC有一定的保护作用。
OBJECTIVE: To investigate if Ruan Tong Xin Dripping Pills have protective effects on rat angiotensin II (Ang II)-induced endothelial injury. METHODS: 125 SD rats were divided into 1, 3, 7, 14 and 28 d groups, 25 in each group, divided into 5 sham-operated groups, 10 in the endothelial injury group and 10 in the musk group. In the control group, a 0.9% sodium chloride solution sustained-release pump was embedded; an AngII slow-release pump was implanted in the endothelial injury group at a dose of 200 ng·min-1·kg-1. The bud group was immersed in the AngII slow-release pump for 3 days before the end of the experiment. Ruan Tongxin Dripping Pills were administered to the stomach (dose 5 mg/kg/d). Animals were sacrificed at 1, 3, 7, 14 and 28 days after operation. Blood endothelin (ET), nitric oxide (NO), and immunoturbidimetric assay for C-reactive protein (CRP) were measured by radioimmunoassay. Tumor necrosis was measured by ELISA. Factor-alpha (TNF-alpha) content. The morphology of the aortic endothelium was observed by scanning electron microscope and the structure of the renal artery endothelium was observed by transmission electron microscopy. RESULTS: In the sham operation group, the endothelium was intact; in the endothelial injury group, endothelial cells (EC) were congested, edematous, and detached, and the internal elastic plates were exposed; whereas in the 1st, 3d, and 7d groups, the musk group was relatively lightly damaged. The 14-day group and the 28-day group had different levels of EC recovery. Conclusion: Qixiang Tongxin Dripping Pills can reduce the contents of ET, CRP and TNF-α in blood, increase the content of NO, and have a certain protective effect on EC.