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目的探讨高压氧对帕金森病大鼠多巴胺神经元的保护作用。方法将68只雌性SpragueDawley大鼠随机分成5组:注射生理盐水高压氧处理组(A组,n=7)、全程高压氧处理模型组(B组,n=18)、未经高压氧处理模型组(C组,n=7)、造模后高压氧处理组(D组,n=18)、造模前高压氧处理组(E组,n=18)。在实验第1天至第7天给予A组、B组和E组大鼠高压氧治疗;而在实验第8天时,分别向B组、C组、D组及E组大鼠单侧脑黑质内定位注射6羟基多巴胺以制作偏侧帕金森病大鼠模型,给予A组等量生理盐水定位注射。从实验第8天至结束,分别给予A组、B组及D组大鼠高压氧处理;并于造模后第9天,16天及21天每组各处死6只大鼠,取其纹状体用分光光度计测定超氧化物歧化酶(SOD)、丙二醛(MDA)和谷胱甘肽过氧化物酶(GSHPx)含量,选用免疫组织化学方法测定黑质区域内酪氨酸羟化酶(TH)阳性细胞数量及胶质纤维酸性蛋白(GFAP)的表达。结果与C组比较,B、D、E组大鼠病变侧纹状体内SOD及GSHPx活性显著增高,MDA含量及GFAP表达明显降低,6羟基多巴胺毁损黑质区残存的TH阳性细胞数目明显增加。结论高压氧治疗可以显著提高机体抗自由基损伤功能、减弱胶质细胞效应发挥,从而有效保护脑黑质区多巴胺(DA)能神经元功能。
Objective To investigate the protective effect of hyperbaric oxygen on dopamine neurons in Parkinson’s disease rats. Methods Sixty-eight female Sprague Dawley rats were randomly divided into five groups: hyperbaric oxygen group (group A, n = 7), hyperbaric oxygen group (group B, n = 18) (Group C, n = 7), hyperbaric oxygen treatment group (group D, n = 18) and model hyperbaric oxygen treatment group (group E, n = 18) The rats in group A, group B and group E were treated with hyperbaric oxygen during the first day to the seventh day of the experiment. On the 8th day of the experiment, rats in group B, group C, group D and group E, Intraperitoneal injection of 6-hydroxydopamine to make a rat model of Parkinson’s disease, group A was given the same amount of saline injection. From the eighth day to the end of the experiment, the rats in group A, group B and group D were treated with hyperbaric oxygen respectively. Six rats in each group were sacrificed on day 9, day 16, and day 21, The contents of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSHPx) were determined by spectrophotometer. The tyrosine hydroxyl content in substantia nigra was detected by immunohistochemistry (TH) positive cells and glial fibrillary acidic protein (GFAP) expression. Results Compared with group C, the activities of SOD and GSHPx in the lateral striatum of rats in groups B, D and E were significantly increased, the content of MDA and the expression of GFAP were significantly decreased, while the number of TH positive cells remaining in the substantia nigra of 6 - hydroxydopamine was significantly increased. Conclusion Hyperbaric oxygen therapy can significantly improve the body against free radical damage, weakened glial cell effect play, so as to effectively protect the dopaminergic neurons in the substantia nigra.