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甲基胆蒽(0.1微克/毫升)不能使C_3H/10T2/1细胞发生转化,但经甲基胆蒽(0.1微克/毫升)启动的细胞,以后用TPA(0.1微克/毫升)连续作用则可以转化。说明TPA具有明显的促进细胞转化作用。TPA不能使经甲基胆蒽启动的细胞的姊妹染色单体交换进一步增加。TPA对C_3H/10T2/1细胞的DNA合成,不论是否先经甲基胆蒽启动,都表现为暂时抑制和以后明显的增强。本文对TPA的促进转化作用及其它有关的生物学作用进行了初步讨论。
Methylcholanthrene (0.1 μg/ml) does not convert C_3H/10T2/1 cells, but cells activated with methylcholanthrene (0.1 μg/ml) can be treated with TPA (0.1 μg/ml) continuously. Conversion. This shows that TPA has a significant role in promoting cell transformation. TPA does not further increase the sister chromatid exchange of cells that are primed by methylcholanthrene. The DNA synthesis of TPA on C_3H/10T2/1 cells, whether or not it was first initiated by methyl-cholesterol, showed a temporary inhibition and later significant enhancement. This article discusses the promotion of transformation and other related biological effects of TPA.