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目的:探索黄连、连翘药对抗炎作用的最佳配伍比例。方法:对黄连、连翘药对不同配伍比例采取测量蛋清致大鼠足趾肿胀的方法进行抗炎研究,分别给予黄连、连翘、各比例药对、阿司匹林以及生理盐水,分别于5,30 min,1,2,4,6 h测量足趾体积,计算肿胀率;采用高效液相色谱法测定各比例药对中咖啡酸、连翘酯苷、芦丁、连翘苷、药根碱、黄连碱、巴马汀、小檗碱含量,计算综合评分。结果:黄连、连翘药对配伍各比例均有一定抑制肿胀的作用,经过分析比例为1∶1的药对各时间点肿胀率最低,抗炎效果最好;将各比例药对按照设定好的权重计算综合评分,得到黄连、连翘药对配伍比例为1∶1时指标性成分的综合评分最高。结论:黄连、连翘药对抗炎作用的最佳配伍比例为1∶1。
Objective: To explore the best proportion of Coptis chinensis and Forsythia suspense for anti-inflammatory effect. Methods: Anti-inflammation was researched on Coptis chinensis and Forsythia suspense to different compatibility ratio to test the swelling of the toe of rat induced by egg white. The Coptis chinensis and Forsythia suspense were respectively administrated with Coptis, Forsythia, Aspirin and Saline, min, 1, 2, 4, 6 h to measure the volume of the toe and calculate the swelling rate; the determination of caffeic acid, forsythiaside, rutin, forsythin, jatrorrhizine, Coptisine, palmatine, berberine content, calculate the comprehensive score. Result: Coptis chinensis and Forsythia suspense had a certain effect of inhibiting the swelling in every proportion. After the ratio of 1: 1 was analyzed, the swelling rate was the lowest and the anti-inflammatory effect was the best at all time points. Good weight to calculate the composite score, get Coptis, forsythia compatibility ratio of 1: 1 when the highest index of the composite score. Conclusion: Coptis chinensis, forsythia on the anti-inflammatory effect of the best compatibility ratio of 1: 1.