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目的 探讨埃兹蛋白(ezrin)在支气管上皮细胞(16HBE)间充质转化(EMT)中的作用.方法 建立转化生长因子-β (TGF-β)诱导的16HBE的EMT模型;RT-PCR和Westernblot法检测16HBE中ezrin mRNA和蛋白表达;通过慢病毒shRNA抑制ezrin表达,观察细胞形态,检测细胞EMT的生物标志分子上皮钙黏着蛋白(E-cadherin)、波形蛋白(vimentin)和α-平滑肌动蛋白(alpha smooth muscle actin,α-SMA)的mRNA和蛋白表达水平;加入外源性ezrin蛋白,细胞免疫荧光、RT-PCR及Western blot法检测其对EMT标志物的影响.结果 TGF-β能下调16HBE中ezrin的mRNA和蛋白表达.经ezrin敲减后,同TGF-β刺激表现相同,16HBE也由典型的铺路石样变为梭形,同时其E-cadherin表达下降,vimentin和α-SMA表达增加;加入重组蛋白ezrin后,16HBE的E-cadherin表达增多,vimentin和α-SMA表达下降.结论 TGF-β可能通过下调ezrin表达促进16HBE发生EMT.“,”Objective To investigate the effects of ezrin on transforming growth factor beta (TGF-β) mediated epithelial mesenchymal transition (EMT).Methods Human bronchial epithelial 16HBE cells were stimulated by TGF-β to induce EMT.The mRNA and protein levels were analyzed by RT-PCR and Western blot.Lentivirus (LV)-Ezrin-shRNA was employed to investigate the effects of ezrin deficiency on cell morphology and expressions of EMT associated biomarkers including E-cadherin,vimentin,and alpha smooth muscle actin (α-SMA).The effects of recombinant ezrin protein on 16HBE were also examined.Results The expression ofezrin was down-regulated in 16HBE activated by TGF-β.Due to ezrin depletion,the cell morphology changed from a typical multilateral paving stone-like appearance to a mesenchymal-like fusiform appearance along with the decreased expression of epithelium biomarker E-cadherin and the increased mesenchymal cell markers,vimentin and αt-SMA.Recombination protein ezrin increased the expression of E-cadherin whilst reducing vimentin and αt-SMA.Conclusion TGF-β promotes EMT of 16HBE cells at least partly via inhibiting the expression of ezrin.