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研究佐剂Al(OH)_3与CpG2216对FI-RSV疫苗增强性免疫病理的不同作用,探寻FI-RSV疫苗增强性免疫病理的发生机制和解决方法,分别制备加入佐剂Al(OH)_3与CpG2216的FI-RSV疫苗,肌注免疫C57BL/6小鼠2次。免疫后观察小鼠发病情况,检测抗体水平、细胞因子转录表达并做肺组织切片等。实验发现两组血清IgG抗体产生无显著差异;FIRSV+CpG2216组小鼠有明显的发病迹象;病理切片显示,FI-RSV+Al(OH)_3组的黏液分泌最明显,FI-RSV+CpG2216组肺部显示更大量炎症细胞浸润,炎症反应较强;FI-RSV+CpG2216组肺组织RSV-N基因的表达量显著高于FI-RSV+Al(OH)_3组(P<0.05),FI-RSV+Al(OH)_3组的IFN-γ、IL-5、TNF-α、GATA-3、T-bet基因的表达量显著高于其他各组(P<0.05),FI-RSV+CpG2216组IL-17、ROR-γt基因的表达量显著高于其他各组(P<0.05)。认为CpG2216与Al(OH)_3单独作为佐剂在诱导抗体产生方面无明显差异;CpG2216单独作为佐剂引发了较强肺部炎症损伤和病理表现,可能因为其诱导了更强的Th17型优势应答所致;Al(OH)_3单独作为佐剂引发了较强的气管黏液分泌和Th2型优势应答。
To investigate the different mechanisms of FI-RSV vaccination on adjuvant Al (OH) _3 and CpG2216, and to explore the mechanism and solution of enhanced immunopathology of FI-RSV vaccine.Methods: CpG2216 FI-RSV vaccine was intramuscularly immunized C57BL / 6 mice twice. Immunological observation of the incidence of mice, detection of antibody levels, expression of cytokines and do lung tissue sections. The results showed that there was no significant difference between the two groups in the serum IgG antibody production; FIRSV + CpG2216 mice had obvious signs of onset; pathological sections showed that mucus secretion was the most obvious in FI-RSV + Al (OH) FI-RSV + CpG2216 group showed significantly higher expression of RSV-N gene in lung tissue than in FI-RSV + Al (OH) 3 group (P <0.05), but FI- The expression of IFN-γ, IL-5, TNF-α, GATA-3 and T-bet in RSV + Al (OH) 3 group were significantly higher than those in other groups (P < IL-17, ROR-γt gene expression was significantly higher than the other groups (P <0.05). CpG2216 and Al (OH) 3 alone were considered as adjuvants in inducing no significant difference in antibody production; CpG2216 alone as an adjuvant elicited strong lung inflammatory lesions and pathological findings probably because it induced a stronger Th17-type predominant response ; Al (OH) _3 alone as an adjuvant elicited strong tracheal mucus secretion and Th2-type predominant response.