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研究糜子苗期抗旱指标,为糜子品种抗旱性鉴定和品种筛选提供理论依据。选用10个不同基因型糜子为材料,通过0.25g/mL聚乙二醇6000(PEG6000)模拟干旱的方法,研究了糜子苗期部分形态指标、生理生化指标与抗旱性的关系。结果表明:水分胁迫下糜子叶片过氧化物酶(POD)活性、丙二醛(MDA)含量、可溶性蛋白(SP)含量以及超氧阴离子自由基(O2-.)明显提高,而株高(PH)、叶长(LL)、叶绿素(Chl)含量、叶片相对含水量(RWC)和超氧化物歧化酶(SOD)活性显著降低。存活率(SR)与以上9个形态、生理生化指标的相关性大小依次为SOD活性>Chl含量>MDA含量>POD活性>O2-.>RWC>LL>PH>SP含量;通过因子分析将10个抗旱性指标用2个公共因子表示,累积贡献率达到92.648%,因子1反映叶绿素含量、活性氧代谢、质膜稳定性及SR对抗旱性起支配作用,因子2中反映糜子生长发育、叶片相对含水量及渗透调节物质对抗旱性起支配作用;灰色关联度分析表明,各抗旱性形态、生理生化指标与SR关联密切程度依次为SOD活性>Chl含量>POD活性>PH>RWC>LL>SP>O2-.>MDA含量。因此,在糜子苗期鉴定中,糜子叶片内抗氧化酶系统指标SOD、POD活性以及Chl含量可作为抗旱性鉴定的有效指标,而LL、PH、SP、MDA含量和O2-.含量可作为参考指标。
Research on drought resistance index of millet seedling stage provide the theoretical basis for drought resistance identification and breed screening of millet varieties. Ten different genotypes of millet were used as materials to study the relationship between morphological, physiological and biochemical indexes and drought resistance of millet at the seedling stage by 0.25g / mL polyethylene glycol 6000 (PEG6000) simulated drought. The results showed that under water stress, the activities of peroxidase (POD), malondialdehyde (MDA), soluble protein (SP) and superoxide anion radical (O2- ), Leaf length (LL), chlorophyll (Chl) content, leaf relative water content (RWC) and superoxide dismutase (SOD) activity were significantly decreased. The correlation between survival rate (SR) and the above nine morphological and physiological indexes was SOD activity> Chl content> MDA content> POD activity> O2-> RWC> LL> PH> SP content. The drought resistance index was expressed by two public factors, the cumulative contribution rate reached 92.648%. The factor 1 reflected chlorophyll content, reactive oxygen metabolism, plasma membrane stability and SR on droughts, while factor 2 reflected the growth and development of millet, Relative water content and osmoregulation substances played a dominant role in drought resistance. Gray correlation analysis showed that the correlations between the morphological, physiological and biochemical indexes of drought resistance and SR were SOD activity> Chl content> POD activity> PH> RWC> LL> SP> O2 -.> MDA content. Therefore, during the seedling stage identification of millet, the activities of SOD and POD and the content of Chl in the leaves of millet were the effective indicators for drought resistance identification, and the content of LL, PH, SP, MDA and O2-. index.