论文部分内容阅读
白羊草幼穗在附加有２，４－Ｄ１．０ｍｇ／Ｌ、水解酪蛋白５００或１０００ｍｇ／Ｌ的Ｎ６或ＭＳ培养基中，在２５±１℃，暗光培养条件下，两周时形成０．５ｃｍ２大小的愈伤组织块。愈伤组织的发生率为８４％。培养３周后形成旺盛生长的愈伤组织。其分化是在含有ＮＡＡ０．５ｍｇ／Ｌ、ＫＴ１．０ｍｇ／Ｌ的ＭＳ培养基上或者在附加有ＫＴ１．０ｍｇ／Ｌ、ＩＡＡ０．４ｍｇ／Ｌ、ＢＡ１．０ｍｇ／Ｌ的Ｎ６培养基上２～４周后均可得到旺盛生长的分化植株，将该植株移至不含激素的Ｎ６培养基内，在光照下进行培养，形成绿色分化植株。３～４周后，将分化植株移入土壤中，植株生长良好。
The young ear of Leymus chinensis was formed in N6 or MS medium supplemented with 2,4-D1.0mg / L, hydrolyzed casein 500 or 1000mg / L at 25 ± 1 ℃ under dark light for two weeks 0.5 cm 2 callus pieces. The incidence of callus was 84%. Three weeks after the formation of strong growth of callus. The differentiation was performed on MS medium containing NAA 0.5 mg / L, KT 1.0 mg / L or N4 medium supplemented with KT 1.0 mg / L, IAA 0.4 mg / L, and BA 1.0 mg / L Well-growing differentiated plants were obtained weeks later, the plants were moved to hormone-free N6 medium and cultured under light to form green-differentiated plants. After 3 to 4 weeks, the differentiated plants were moved into the soil and the plants grew well.