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该研究旨在建立联体小鼠模型并评估小鼠应对联体状态时的生理变化。选取13对同基因联体小鼠,在两只小鼠肘关节远端4～5mm处,沿着肱骨和肋骨侧面直到腰围线末端,连接皮肤和皮下组织,建立联体模型,术后观察联体小鼠运动变化、体重、粪便皮质酮活性及血液交换率等。结果发现:术后联体小鼠出现兴奋、焦虑等特征性运动失常,随后数周小鼠活动逐渐适应联体状态,120天内无一死亡;术后三天体重下降,术后一月恢复至正常;术后粪便中皮质酮代谢物水平快速升高,至第75天降至正常;检测术后其中三对联体小鼠之间的血液交换率,结果基本正常,第12周血液完全交换率分别为63、46和107min。该结果提示小鼠能够很好地适应联体状态,该模型具有广泛的应用前景。
The aim of the study was to establish a linked mouse model and to evaluate the physiological changes that mice experience when they are linked. 13 pairs of syngeneic mice were selected to establish a conjoined model at 4 to 5 mm distal to the elbow of the two mice along the humerus and the lateral side of the rib until the end of the waistline, Body movement changes in mice, body weight, fecal corticosterone activity and blood exchange rate. The results showed that the conjunctive mice showed characteristic motor disorders such as excitement and anxiety. After a few weeks, the mice gradually adapted to the conjunctive state without any death within 120 days. The body weight decreased three days after the operation and recovered to Normal; postoperative corticosterone metabolites in the stool rapidly increased to 75 days to normal; test three pairs of conjoined mice after the blood exchange rate, the results were normal, the first 12 weeks of complete exchange rate of blood Respectively 63,46 and 107min. The results suggest that mice can well adapt to the conjoint state, the model has a wide range of applications.