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漆酶在食用菌生长过程中具有降解木质素、影响子实体形成等重要生理功能,而营养条件会显著影响酶的形成。本研究克隆了杏鲍菇和松杉灵芝部分漆酶基因,比较分析了不同营养条件对漆酶基因表达量及胞外酶活的影响。结果表明,两种食用菌漆酶基因序列差异较大,蛋白同源性低;杏鲍菇在高N低C、高无机盐、完全培养基中胞外漆酶酶活较高,而在转录水平基因表达量高低分别为低N、完全、高N低C培养基;加入有机碳如蔗渣在促进松杉灵芝漆酶基因的转录同时可提高胞外酶活,同时Cu2+可诱导漆酶的分泌,但无诱导漆酶转录作用。C、N比例的调整对2种食用菌漆酶基因的表达与胞外酶活影响具有明显差异,高氮源可促进菌株漆酶的分泌。该结果为进一步探讨漆酶基因调控机制及其生物学功能提供理论基础,同时为改变营养条件提高食用菌胞外漆酶酶活,加强食用菌胞外漆酶的应用提供理论依据。
Laccase in the process of mushroom growth with lignin degradation, affecting the formation of fruiting bodies and other important physiological functions, and nutrition will significantly affect the formation of enzymes. In this study, part of laccase genes of Pleurotus eryngii and Ganoderma lucidum were cloned, and the effects of different nutrient conditions on laccase gene expression and extracellular enzyme activity were compared. The results showed that there were significant differences in the sequences of the two edible fungi laccase genes and low homology of proteins. Pleurotus eryngii had higher activity of extracellular laccase in high N and low C, high inorganic salts and complete medium, High and low level of gene expression was low N, complete, high and low N medium C; addition of organic carbon such as bagasse promoted the translocation of Ganoderma lucidum laccase gene can increase extracellular enzyme activity, while Cu2 + can induce the secretion of laccase , But did not induce laccase transcription. C, N ratio adjustment of the two kinds of mushroom laccase gene expression and extracellular enzyme activity have significant differences, high nitrogen source can promote bacterial laccase secretion. The results provide a theoretical basis for further study on the regulation mechanism of laccase gene and its biological function, and provide theoretical basis for changing the nutritional conditions to improve the enzyme activity of extracellular laccase of edible fungi and to strengthen the application of extracellular laccase of edible fungi.