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研究了超低温冷冻保存(-196℃)对罗氏沼虾胚胎内总三磷酸腺苷酶(ATPase)、肌酸激酶(CK)、琥珀酸脱氢酶(SDH)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)等酶活性的影响。运用试剂盒分别测定了冷冻前后罗氏沼虾胚胎内酶活性的变化。结果表明,经过超低温冷冻保存后,胚胎内7种酶的活性均显著下降(P<0.05),其中总ATPase、CK、LDH和SDH的活性分别从冻前的1.322±0.162 U/mg prot、0.404±0.015 U/mg prot、352.225±23.214 U/gprot和2.067±0.139 U/mg prot下降到0.087±0.003 U/mg prot、0.010±0.002 U/mg prot、5.890±0.658 U/gprot和0.552±0.138 U/mg prot;SOD、CAT和GSH-Px的活性分别从冻前的19.217±0.677 U/mg prot、3.587±0.233 U/mg prot和7.626±1.106 U/(min.mg)下降至3.579±0.234 U/mg prot、1.773±0.227 U/mg prot和1.524±0.096 U/(min.mg);MDA的活性从1.015±0.038 n mol/mg prot上升到20.937±0.320 n mol/mgprot,超低温冷冻对罗氏沼虾胚胎酶活性有显著性影响。
The effects of cryopreservation (-196 ℃) on total ATPase, creatine kinase (CK), succinate dehydrogenase (SDH), lactate dehydrogenase (LDH), superoxide The activities of SOD, CAT, GSH-Px and MDA in the leaves of the two groups were measured. The changes of enzyme activities in embryos of Macrobrachium rosenbergii before and after freezing were measured by kit. The results showed that after cryopreservation, the activities of seven enzymes in embryos decreased significantly (P <0.05), and the activities of total ATPase, CK, LDH and SDH decreased from 1.322 ± 0.162 U / mg prot, 0.404 ± 0.015 U / mg prot, 352.225 ± 23.214 U / gprot and 2.067 ± 0.139 U / mg prot decreased to 0.087 ± 0.003 U / mg prot, 0.010 ± 0.002 U / mg prot, 5.890 ± 0.658 U / gprot and 0.552 ± 0.138 U / mg prot; the activities of SOD, CAT and GSH-Px decreased from 19.217 ± 0.677 U / mg prot, 3.587 ± 0.233 U / mg prot and 7.626 ± 1.106 U / (min.mg) before freezing to 3.579 ± 0.234 U / mg prot, 1.773 ± 0.227 U / mg prot and 1.524 ± 0.096 U / (min.mg), respectively. The MDA activity increased from 1.015 ± 0.038 n mol / mg prot to 20.937 ± 0.320 n mol / mg prot. Shrimp embryo enzyme activity has a significant impact.