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用化学发光法观测了维拉帕米对人血ＰＭＮ细胞受ＰＭＡ刺激发生呼吸暴发产生的活性氧，黄嘌呤－黄嘌呤氧化酶体系产生的Ｏ，ＶｉｔＣ－Ｃｕ￣（２＋）－酵母多糖体系产生的·ＯＨ，及Ｈ＿２Ｏ＿２的释放反应的影响。结果表明，维拉帕米对ＰＭＮ细胞的呼吸暴发全过程均有显著的抑制作用：对Ｏ有清除作用；对Ｈ＿２Ｏ＿２的释放反应有抑制作用，并呈剂量依赖性，ＩＣ＿（５０）分别为１３６ｎｍｏｌ／Ｌ，２８．１ｎｍｏｌ／Ｌ和２２．３ｎｍｏｌ／Ｌ；对·ＯＨ则无作用。
The chemiluminescence method was used to observe the effect of verapamil on the reactive oxygen species (ROS) produced by respiratory burst of PMMA stimulated by PMA in human blood. O, VitC-Cu ~ (2 +) - zymosan system produced by xanthine-xanthine oxidase system · OH and H 2 O 2 release reactions. The results showed that verapamil had a significant inhibitory effect on the whole process of respiratory burst in PMN cells: scavenging O; inhibiting the release of H 2 O 2 in a dose-dependent manner with IC50 of 136 nmol /L,28.1nmol/L and 22.3nmol / L; no effect on · OH.