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诱导体外培养扩增后的小鼠骨髓ＣＤ３４＋造血细胞分化为树突状细胞（ＤＣ）。方法Ｃ５７ＢＬ／６ｊ小鼠骨髓ＣＤ３４＋细胞采用血管内皮细胞共培养方法进行体外扩增，扩增后的ＣＤ３４＋细胞经磁性细胞分选技术纯化，并用ＧＭ┐ＣＳＦ等细胞因子诱导其向ＤＣ分化。结果小鼠骨髓ＣＤ３４＋造血细胞不论是否经过体外培养均可被诱导分化为ＤＣ，后者具有典型的树突状形态特征，表达高水平的ＭＨＣⅠ、Ⅱ类抗原和ＣＤ８６共刺激分子，在混合白细胞反应（ＭＬＲ）中能有效地刺激静息期Ｔ淋巴细胞增殖。结论小鼠骨髓ＣＤ３４＋细胞经体外培养扩增后仍然具有向ＤＣ分化的能力，通过骨髓细胞体外培养扩增可以显著提高ＤＣ产量。
In vitro cultured mouse bone marrow CD34+ hematopoietic cells were induced to differentiate into dendritic cells (DCs). Methods C57BL/6j mouse bone marrow CD34+ cells were expanded in vitro using vascular endothelial cell co-culture method. The amplified CD34+ cells were purified by magnetic cell sorting technique and induced to differentiate into DCs with GM┐CSF and other cytokines. RESULTS: Mouse bone marrow CD34+ hematopoietic cells could be induced to differentiate into DC whether or not they were cultured in vitro. The latter had typical dendritic morphological features, expressing high levels of MHC class I, class II antigens, and CD86 costimulatory molecules in a mixed leukocyte response. (MLR) can effectively stimulate resting T lymphocyte proliferation. Conclusion The mouse bone marrow CD34+ cells still have the ability to differentiate into DC after being cultured in vitro. The expansion of bone marrow cells in vitro can significantly increase DC production.