[Objective] To investigate the tumor inhibition effects of paclitaxel long-circulating thermo-sensitive liposome( PLTL) on mice bearing Lewis lung carcinoma cells. [Methods] A tumor-bearing mice model was established and divided into five groups randomly,which were control group,heating group,paclitaxel( PTX) group,paclitaxel thermo-sensitive liposome( PTL) group and PLTL( long-circulating thermo-sensitive liposome) group. Local hyperthermia was adopted; the survival status of mice was observed; volume and weight of tumor were detected; HE staining of tumor section was carried out; morphological changes of tumor cells were observed; and cell apoptosis was detected by flow cytometry. [Results]Tumor inhibition rates of PTX,PTL and PLTL groups were 48. 87%,57. 22% and 78. 87%,respectively. And the cell apoptosis rates were( 42. 7 ± 3. 8) %,( 54. 6 ± 2. 9) % and( 69. 7 ± 5. 0) %,respectively. [Conclusions] Compared with PTX and PTL,PLTL had evident thermo-sensitive characteristics,and increased the anticancer effect of paclitaxel remarkably when combined with local hyperthermia. [Objective] To investigate the tumor inhibition effects of paclitaxel long-circulating thermo-sensitive liposome (PLTL) on mice bearing Lewis lung carcinoma cells. [Methods] A tumor-bearing mice model was established and divided into five groups randomly, which were control group, heating group, paclitaxel (PTX) group, paclitaxel thermo-sensitive liposome (PTL) group and PLTL (long-circulating thermo-sensitive liposome) group. Local hyperthermia was adopted; the survival status of mice was observed; volume and weight of Tumor inhibition rates of PTX, PTL and PLTL groups were 48. 87%, 57 Respectively. And the cell apoptosis rates were (42.7 ± 3.8)%, (54.6 ± 2.9)% and (69.7 ± 5.00)%, respectively respectively. [Conclusions] Compared with PTX and PTL, PLTL had evident thermo-sensitive characteristics, and i ncreased the anticancer effect of paclitaxel remarkably when combined with local hyperthermia.