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目的观察RNT4蛋白在胃癌组织中的表达及意义,为获取胃癌诊断相关的蛋白分子提供实验依据。方法采用RT-PCR和Western blot检测RTN4在胃癌组织中的表达;应用免疫组化染色检测胃癌组织中RTN4蛋白的表达率,并分析其表达的临床意义。结果 1RT-PCR和Western blot结果均显示,与正常胃黏膜组织比较,RTN4在胃癌组织中的表达明显增加(P<0.05)。2胃癌与正常胃黏膜组织中RTN4蛋白的阳性表达率分别为60.9%(28/46)与25%(6/24),RTN4蛋白在胃癌组织中阳性表达率显著高于正常胃黏膜组织(P<0.05)。结论 RNT4蛋白在胃黏膜组织中高表达与胃癌的发生密切相关。
Objective To observe the expression and significance of RNT4 protein in gastric cancer tissues and provide experimental evidence for obtaining protein molecules related to gastric cancer diagnosis. Methods The expression of RTN4 in gastric cancer tissues was detected by RT-PCR and Western blot. The expression of RTN4 protein in gastric cancer tissues was detected by immunohistochemistry, and its clinical significance was analyzed. Results The results of 1RT-PCR and Western blot showed that compared with normal gastric mucosa, the expression of RTN4 in gastric cancer tissues was significantly increased (P <0.05). The positive expression rates of RTN4 protein in gastric cancer and normal gastric mucosa were 60.9% (28/46) and 25% (6/24), respectively. The positive rate of RTN4 protein in gastric cancer was significantly higher than that in normal gastric mucosa <0.05). Conclusion The high expression of RNT4 protein in gastric mucosa is closely related to the occurrence of gastric cancer.