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为研究四氯化碳急性肝损伤后各项生化指标及病理损伤的时间—反应关系 ,选用Wistar种雄性大鼠 ,随机分为 5组 ,每组 10只。试验组动物给予 2 %CCl4 ,灌胃 160mg/kgBW ,阴性对照组给予植物油。分别于染毒后 12、2 4、4 8、72h(对照组给油后 2 4h)处死动物取静脉血测定血清GPT、GOT、TG含量 ,大鼠染毒后血清GPT、GOT含量显著性升高 ,2 4h达到峰值后逐渐下降 ,72h接近正常 ;12h肝组织中MDA含量显著性升高 ;GSH含量 12～ 4 8h显著性升高。血清TG无明显变化 ,肝组织中TG含量 12h显著性升高 ,4 8h后恢复正常。染毒后 12、2 4、4 8h各组大鼠肝脏均出现典型四氯化碳中毒的病理变化 ,表明检测四氯化碳所致肝损伤可在染毒后 12～ 4 8h测定血清GPT、GOT ,进行肝脏病理组织学检查。此外 ,肝组织中MDA、GSH和TG含量的测定也可作为检测的辅助指标 ,有助于评价保健食品的保肝功能。
In order to study the time-response relationship of biochemical parameters and pathological injury after CCl 4 acute liver injury, Wistar male rats were randomly divided into 5 groups with 10 in each group. Animals in the experimental group were given 2% CCl4, gavage 160mg / kg BW, and the negative control group was given vegetable oil. The levels of GPT, GOT and TG in blood were measured at 12, 24, 48, and 72h after the rats were sacrificed (control group, 24 hours after oiling). Serum GPT and GOT levels were significantly increased High, peaked at 24 hours, then decreased gradually, and reached the normal level at 72 hours. The content of MDA in liver tissue increased significantly at 12 hours and the content of GSH increased significantly at 12 ~ 48 hours. No significant changes in serum TG, TG content in liver tissue significantly increased 12h, return to normal after 48h. The pathological changes of typical carbon tetrachloride poisoning occurred in the livers of rats in each group at 12,2,4,48h after exposure. It was indicated that the detection of serum levels of GPT by carbon tetrachloride-induced hepatic injury at 12 ~ 48 h after exposure, GOT for liver histopathology. In addition, the determination of MDA, GSH and TG content in liver tissue can also be used as an auxiliary indicator of the test to help evaluate the liver protection function of health food.