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建立了反相高效液相色谱测定元胡止痛胶囊中欧前胡素含量的方法。采用乙醇作溶剂，水浴加热回流提取。用 ＬＵＮＡ－Ｃ_１８色谱柱分离测定，流动相为甲醇－０．５％磷酸（６９∶３１），流速０．８ｍＬ／ｍｉｎ，检测波长 ３００ｎｍ。欧前胡素与其相邻峰的分离度是 ２．０，理论板数按欧前胡素峰计算是１５３６７，欧前胡素对照品线性范围 １９．５０～５８．５０μｇ／ｍＬ， ｒ＝０．９９９９，平均回收率９９．８％。本法操作简便，结果准确。
A method was established for the determination of imperatorin in Yuanhu Zhitong Capsules by RP-HPLC. Using ethanol as solvent, the water bath was heated to reflux extraction. The mobile phase was methanol-0.5% phosphoric acid (69:31) with a flow rate of 0.8 mL / min and the detection wavelength was 300 nm. The separation of imperatorin and its adjacent peak is 2.0, the theoretical plate number is 15367 calculated by imperatorin, and the linear range of imotenumab reference 19.50 ~ 58.50μg / mL, r = 0.9999 , The average recovery rate of 99.8%. This method is simple and accurate.