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Aerobic granular sludge were cultivated in sequencing batch airlift reactors (SBAR) at 25, 30 and 35℃,respectively. The effects of temperature on the granules characteristics were analyzed and the microbial community structures of the granules were probed using scanning electron micrograph (SEM) and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). The results showed that 30℃ is optimum for matured granules cultivated, where the granules had more compact structure, better settling ability and higher bioactivity, the OUR reached to 1.14 mg O2/(g MLVSS·min) with 97% removal rate of COD and 75% removal rate of TP, and the removal efficiency of NH3-N increased along with the temperature rising in the range of 68.5%-87.5%. DGGE profiles revealed that microbial community structure at 25℃ showed the lowest similarity with others, the sequencing results indicated that most of dominant microbes belonged to Actinobacteria and Proteobacterium. Thermomonas sp., Ottowia sp. and Curtobacterium ammoniigenes may act important roles respectively at different temperature.