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目的对北京地区2004年11月~2005年3月流行性感冒患儿进行病原学检测及分析。方法狗肾传代细胞(MDCK)进行流感病毒分离,用血凝试验、血凝抑制试验进行型和亚型的鉴定;用快速酶联免疫法检测A型及B型流感病毒抗原试剂盒检测患儿咽拭子中的流感病毒抗原。结果217例患儿中111例检测出流感病毒,检出率为51%(111/217),流感病毒A型28例(28/111),占检出数的25·3%,流感病毒B型82例(82/111),占检出数的73·8%,流感病毒A+B型1例(1/111),占检出数的0·9%。111例流感病毒检测阳性患儿中病毒分离与快速诊断二项同时阳性75例,二者阳性符合率67·5%。结论北京地区2004年11月~2005年3月流感流行季节流感病毒以B型为优势株。流感快速诊断试剂盒可快速、准确地检测出流感病毒及型别。
Objective To detect and analyze the etiology of children with influenza from November 2004 to March 2005 in Beijing. Methods MDCK was isolated from influenza virus by hemagglutination test and hemagglutination inhibition test. The rapid and enzyme-linked immunosorbent assay was used to detect influenza A and B influenza virus antigen in children Flu virus antigen in throat swab. Results Of the 217 cases, 111 cases were detected with influenza virus, the detection rate was 51% (111/217), influenza virus A type 28 cases (28/111), accounting for 25.3% of the detection number, influenza virus B 82 cases (82/111), accounting for 73.8% of the detected cases, and 1 case (1/111) of influenza virus A + B cases, accounting for 0.9% of the detected cases. Among the 111 positive cases of influenza virus, 75 cases were positive for virus isolation and rapid diagnosis, the positive coincidence rate was 67.5%. Conclusions In November 2004 to March 2005 in Beijing, Influenza A was the predominant influenza strain in the seasonal flu season. Flu rapid diagnostic kit for rapid and accurate detection of influenza virus and type.