论文部分内容阅读
目的:建立人喉鳞癌(Hep-2)裸鼠移植模型,比较两种建模方法成瘤效果。方法:将裸鼠随机分成两组,每组8只,两组分别于皮下接种人喉鳞癌细胞悬液和改良法皮下接种瘤块建立动物模型,观察接种成瘤率、肿块大小、生长速度;免疫组化检测人端粒酶逆转录酶(hTERT)蛋白表达。结果:接种3d后,改良瘤块接种法组裸鼠可见皮下隆起瘤体,接种1周后,皮下接种瘤细胞法和改良瘤块接种法成瘤率分别为75%、87.5%,比较第1周瘤体体积,P<0.05,但成瘤后肿瘤生长方面无明显的差别(比较第3周瘤体体积,P>0.05),两种方法成瘤后的瘤块hTERT蛋白表达阳性率相同。结论:采用改良瘤块接种法成瘤率高,生长速度快,且裸鼠皮下肿瘤较好的继承了人喉鳞癌细胞的重要生物学特点,该法能较好建立了人喉鳞癌裸鼠移植模型,为后续以端粒酶为靶点的体内抗肿瘤研究奠定良好基础。
Objective: To establish a human nude mice model of laryngeal squamous cell carcinoma (Hep-2) and compare the tumorigenicity of the two methods. Methods: Nude mice were randomly divided into two groups of 8 rats. The two groups were subcutaneously inoculated with human laryngeal squamous cell carcinoma cell suspension and inoculated subcutaneously with tumor patches to establish an animal model. The tumorigenic rate, tumor size, growth rate The expression of human telomerase reverse transcriptase (hTERT) protein was detected by immunohistochemistry. Results: After 3 days of vaccination, subcutaneously elevated tumors were observed in the nude mice inoculated with the improved tumor inoculation method. One week after inoculation, the tumorigenic rates of subcutaneously inoculated tumor cells and modified tumor inoculation method were 75% and 87.5%, respectively (P> 0.05). The positive rates of hTERT protein expression in the tumor nodules by the two methods were the same. Conclusion: The improved tumor mass inoculation method has the advantages of high rate of tumorigenesis and rapid growth, and the subcutaneous tumor of nude mice inherits the important biological characteristics of human laryngeal squamous cell carcinoma. This method can well establish the human laryngeal squamous cell carcinoma nude Mouse transplantation model for the follow-up telomerase as a target in vivo antitumor research and lay a good foundation.